Iranian Journal of Basic Medical Sciences، جلد ۲۱، شماره ۹، صفحات ۸۸۴-۸۸۸
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody
چکیده انگلیسی مقاله Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-GFP), as a reporter gene, was amplified by PCR and then sub-cloned in the pHEN4/3VGR19 phagemid. The resulting construct was transfected into 293KDR cell. The recombinant phage was extracted and confirmed and then transduced into VEGFR2 expressing cell (293KDR). Results: Seventy-two hr after transfection, green fluorescence was detected in 30% of the cells. About 1% of the cells which transduced by recombinant phages were able to express GFP. Conclusion: It is hoped that the results from this study will help to find potential vectors to improve the efficiency of gene delivery. Taken together, we conclude that this newly-introduced vector can be used in cancer researches.
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نویسندگان مقاله | Farideh Ranjibar
Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran


| Mahdi Habibi-Anbouhi
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran


| Fatemeh Kazemi-Lomedasht
Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran


| Seyed Hamid Aghaee-Bakhtiyari
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Medical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran


| Ehsan Alirahimi
Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran


| Mahdi Behdani
Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_11206.html
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کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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