چکیده انگلیسی مقاله |
Background: Ketamine is a commonly used intravenous anesthetic which produces dissociation anesthesia, analgesia, and amnesia. The mechanism of ketamine-induced synaptic inhibition in high-level cortical areas is still unknown. We aimed to elucidate the effects of different concentrations of ketamine on the glutamatergic synaptic transmission of the neurons in the primary somatosensory cortex by using the whole-cell patch-clamp method. Methods: Sprague-Dawley rats (11–19 postnatal days, n=36) were used to obtain brain slices (300 μM). Spontaneous excitatory postsynaptic currents (data from 40 neurons) were recorded at a command potential of -70 mV in the presence of bicuculline (a competitive antagonist of GABAA receptors, 30 μM) and strychnine (glycine receptor antagonist, 30 μM). Miniature excitatory postsynaptic currents (data from 40 neurons) were also recorded when 1 μM of tetrodotoxin was added into the artificial cerebrospinal fluid. We used GraphPad Prism5for statistical analysis. Significant differences in the mean amplitude and frequency were tested using the Student paired 2-tailed t test. Values of P< 0.05 were considered significant. Results: Different concentrations of ketamine inhibited the frequency and amplitude of the spontaneous excitatory postsynaptic currents as well as the amplitude of the miniature excitatory postsynaptic currents in a concentration-dependent manner, but they exerted no significant effect on the frequency of the miniature excitatory postsynaptic currents. Conclusion: Ketamine inhibited the excitatory synaptic transmission of the neurons in the primary somatosensory cortex. The inhibition may have been mediated by a reduction in the sensitivity of the postsynaptic glutamatergic receptors. |
نویسندگان مقاله |
chengdong yuan | chengdong yuan guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
yajun زانگ | yajun zhang guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
yu زانگ | yu zhang guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
song cao | song cao guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
yuan ونگ | yuan wang guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
bao fu | bao fu guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
tian yu | tian yu guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
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