| چکیده انگلیسی مقاله |
Objective(s): Adipose tissue-derived mesenchymal stem cells (AT-MSCs) with more potent immunomodulatory effects, greater proliferative potential and secretion of growth factors and cytokines in comparison with bone marrow derived MSCs are more appropriate for cell therapy. The aims of the present study were to evaluate the histomorphometric effect of AT-MSCs allotransplantation on regeneration of germinal layer cells of seminiferous tubules in busulfan-induced azoospermic hamsters.Materials and Methods: In the present experimental case-control study, AT-MSCs were isolated from adipose tissue of two female and six male donor albino hamsters, and testes of the males were simultaneously used as negative control group. Six mature male recipient hamsters received two doses of busulfan with three weeks interval to stop endogenous spermatogenesis. Right testis of hamsters was intratubular injected with AT-MSCs via efferent duct 35 days after induction of azoospermia and was used as cell therapy group. The left testis without cell therapy was served as azoospermia group.Results: After 35 days, testes and epididymis in all groups were removed for histological evaluation. Histomorphometric analyses of AT-MSCs-treated testes and epididymis showed that the epithelial tissue of seminiferous tubules was normally repaired in most cell-treated seminiferous tubules, and spermatozoa were present in epididymis tubes in comparison with intact testes. The untreated seminiferous tubules and epididymis tubes of azoospermia group were empty.Conclusion: Allotransplanted AT-MSCs could successfully induce spermatogenesis in azoospermic seminiferous tubules of hamster. Therefore, AT-MSCs can be suggested as an attractive candidate in cell transplantation of azoospermia. |
| نویسندگان مقاله |
| Negar Karimaghai
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| Amin Tamadon
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| Farhad Rahmanifar
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
| Davood Mehrabani
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| Alireza Raayat Jahromi
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
| Shahrokh Zare
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| Zahra Khodabandeh
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| Iman Razeghian Jahromi
Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
| Omid Koohi-Hosseinabadi
Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
| Mehdi Dianatpour
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran|Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
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