| چکیده انگلیسی مقاله |
Objective(s): To elucidate the effects and potential mechanisms of hypericin-photodynamic therapy (HYP-PDT) for treating the human rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) MH7A cell-line. Materials and Methods: MH7A cells were subjected to HYP-PDT intervention and apoptosis was evaluated via MTT, nuclear staining, and flowcytometry analyses. Intracellular reactive oxygen species (ROS) were measured with the fluorescent probe 2'7'-dichlorofluorescein diacetate (DCFH-DA). To verify the effects of HYP on apoptotic and nuclear factor kappa-B (NF-κB) pathways, caspase-8, 9, poly-ADP-ribose polymerase (PARP), phosphorylated (p)-NF-κB p65, NF-κB p65 and p-IκBα protein expressions were quantified with western blot. Quantitative real-time PCR was used to assay NF-κB p65 mRNA. Results: HYP-PDT inhibited MH7A cell viability and induced apoptosis in a dose-dependent manner. Meanwhile, intracellular ROS levels increased significantly after HYP-PDT treatment. Furthermore, the expression of cleaved caspase-9 and PARP was increased by HYP-PDT treatment, with a concurrent decline in NF-κB. Conclusion: HYP-PDT induces apoptosis in MH7A cells, at least partially, via generation of ROS, regulation of the apoptotic pathway and suppression of the NF-κB pathway. These findings suggest that HYP-PDT may be a potential treatment for RA. |
| نویسندگان مقاله |
kun زانگ | kun zhang
department of orthopedics, no. 91 central hospital of liberation army, jiaozuo 454150, henan province, china
shan امتیاز | shan gao
faculty of graduate studies, hunan university of chinese medicine, changsha 410208, hunan province, china
jiayi گیو | jiayi guo
department of orthopedics, luoyang orthopedic hospital of henan province, luoyang 471000, henan province, china
guohua ni | guohua ni
department of orthopedics, no. 91 central hospital of liberation army, jiaozuo 454150, henan province, china
zhe chen | zhe chen
department of orthopedics, luoyang orthopedic hospital of henan province, luoyang 471000, henan province, china
feng li | feng li
department of orthopedics, luoyang orthopedic hospital of henan province, luoyang 471000, henan province, china
xiaolei zhu | xiaolei zhu
department of orthopedics, luoyang orthopedic hospital of henan province, luoyang 471000, henan province, china
yongbing wen | yongbing wen
department of orthopedics, luoyang orthopedic hospital of henan province, luoyang 471000, henan province, china
yanxing گیو | yanxing guo
department of orthopedics, luoyang orthopedic hospital of henan province, luoyang 471000, henan province, china
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