| چکیده انگلیسی مقاله |
Objective(s): Detection of circulating Mycobacterium tuberculosis (M. tuberculosis) antigens is promising in Tuberculosis (TB) diagnosis. However, not a single antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal antibodies targeting multiple antigen markers (multi-target antibodies) and evaluate their efficacies in TB diagnosis. Materials and Methods: A fusion gene consisting of 38kD, ESAT6, and CFP10 was constructed and overexpressed. The fusion polyprotein was used as an immunogen to elicit production of multi-target antibodies. Their reactivities were tested. Then, the multi-target antibodies and three corresponding antibodies elicited by each single antigen (mono-target antibodies) were evaluated with sandwich ELISA for detecting M. tuberculosis antigens. Their diagnostic efficacies for TB were also compared. Results: The polyprotein successfully elicited production of multi-target antibodies targeting 38kD, ESAT6, and CFP10 as analyzed by Western blotting. When used as coating antibodies, the multi-target antibodies were more efficient in capturing the three antigens than the corresponding mono-target antibodies. By testing clinical serum, the multi-target antibodies demonstrated significantly higher sensitivity for clinical TB diagnosis than all three mono-target antibodies. Conclusion: The multi-target antibodies allowed detecting multiple antigens simultaneously and significantly enhanced TB detection compared to routine mono-target antibodies. Our study may provide a promising strategy for TB diagnosis. |
| نویسندگان مقاله |
zhenhua دایی | zhenhua dai
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
zhiqiang liu | zhiqiang liu
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
bingshui xiu | bingshui xiu
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
xiqin یانگ | xiqin yang
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
ping zhao | ping zhao
chaoyang district centre for disease control and prevention, 25 panjiayuan huaweili, beijing 100029, china
xuhui زانگ | xuhui zhang
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
cuimi duan | cuimi duan
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
haiping que | haiping que
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
heqiu زانگ | heqiu zhang
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
xiaoyan feng | xiaoyan feng
department of bio-diagnosis, beijing institute of basic medical sciences, 27 taiping road, beijing 100850, china
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