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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۵، صفحات ۵۹۴-۶۰۳
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| عنوان انگلیسی |
Berberine protects against glutamate-induced oxidative stress and apoptosis in PC12 and N2a cells |
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| چکیده انگلیسی مقاله |
Objective(s): Neurodegenerative diseases have been associated with glutamatergic dysfunction. Berberine, an isoquinoline alkaloid broadly present in different medicinal herbs, has been reported to have neuroprotective effect. In the present study, the effects of berberine against glutamate-induced oxidative damage and apoptosis were investigated. Materials and Methods: The cultured PC12 and N2a cells were pretreated (2 hr) with varying concentrations of berberine (50-1000 µM), followed by exposure to glutamate (10 mM) for 24 hr. The cells viability, intracellular reactive oxygen species (ROS), lipid peroxidation, glutathione (GSH) content, superoxide dismutase (SOD) activity, DNA fragmentation and the expressions of pro-apoptotic (cleaved caspase-3 and bax) and anti-apoptotic (bcl-2) proteins were then measured. Results: In both cell lines, pretreatment with berberine (especially at low concentrations) significantly decreased ROS generation, lipid peroxidation, and DNA fragmentation, while improving glutathione content and SOD activity in glutamate-injured cells. Moreover, berberine showed anti-apoptotic effects by reducing the glutamate-evoked caspase-3 and bax/bcl-2 overexpression. Conclusion: The results of present study suggest that berberine protects against glutamate-induced PC12 and N2a cells injury by decreasing oxidative stress and subsequently inhibiting apoptosis. This is relevant to berberine treatment in neurodegenerative disorders, such as dementia (Alzheimer's disease), seizures, and stroke. |
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| نویسندگان مقاله |
حمیدرضا صادق نیا | hamid reza sadeghnia
division of neurocognitive sciences, psychiatry and behavioral sciences research center, mashhad university of medical sciences, mashhad, iran|pharmacological research center of medicinal plants, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
منیره kolangikhah | monireh kolangikhah
pharmacological research center of medicinal plants, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
الهام اسدپور | elham asadpour
anesthesiology and critical care research center, shiraz university of medical sciences, shiraz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)
فاطمه فروزانفر | fatemeh forouzanfar
pharmacological research center of medicinal plants, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
حسین حسین زاده | hossein hosseinzadeh
pharmaceutical research center, mashhad university of medical sciences, mashhad, iran|department of pharmacodynamics and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_8847.html |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Barberry special issue |
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