Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۵، صفحات ۴۶۷-۴۷۳

عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله

عنوان انگلیسی One-step purification and characterization of alginate lyase from a clinical Pseudomonas aeruginosa with destructive activity on bacterial biofilm
چکیده انگلیسی مقاله Objective(s): Pseudomonas aeruginosais a Gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype. Mucoid strains secrete alginate, which is the main agent of biofilms in chronic P. aeruginosa infections, show high resistance to antibiotics; consequently, the biological disruption of mucoid P. aeruginosa biofilms is an attractive area of study for researchers. Alginate lyase gene (algl) is a member of alginate producing operon which by glycosidase activity produces primer for other enzymes in this cluster. Also this activity can destroy the extracellular alginate; therefore this enzyme participates in alginate production and destruction pathway. Alginate lyase causes detachment of a biofilm by reducing its adhesion to the surfaces, and increases phagocytosis and antibiotic susceptibility. In this study, alginate lyase was purified in just one step and its properties were investigated. Materials and Methods: The purification was done by affinity chromatography, analysed by SDS-PAGE, and its effect on P. aeruginosa biofilms was surveyed by micro titer plate assay and SEM. The substrate specificity of the enzyme was determined by PCR. Results: Alginate lyase from isolate 48 was purified in one step. It is more thermally resistant than alginate lyase from Pseudomonas aeruginosa PAO1 and poly M, poly G and poly MG alginate were the substrate of this enzyme. Moreover, it has an eradication effect on biofilms from P. aeruginosa 48 and PAO1. Conclusion: In this study an alginate lyase with many characteristics suitable in medicine such as thermal stability, effective on poly M alginate, and bacterial biofilm destructive was introduced and purified.
کلیدواژه‌های انگلیسی مقاله

نویسندگان مقاله پریناز قدم | parinaz ghadam
department of biotechnology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

فاطمه اخلاقی | fatemeh akhlaghi
department of biotechnology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

احیا عبدی علی | ahya abdi ali
department of microbiology, faculty of biological sciences, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)


نشانی اینترنتی http://ijbms.mums.ac.ir/article_8668.html
فایل مقاله دریافت فایل مقاله
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات