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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۹، صفحات ۱۰۵۰-۱۰۵۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Presenting a rapid method for detection of Bacillus cereus, Listeria monocytogenes and Campylobacter jejuni in food samples |
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| چکیده انگلیسی مقاله |
Objective(s): Listeria monocytogens, Bacillus cereus and Campylobacter jejuni are three toxin producing bacteria over the world, especially in Iran, and it is essential to find a certain, rapid procedure to identify these microorganisms. In this research, these bacteria were simultaneously detected by multiplex PCR technique in foods. Materials and Methods: The primary approval of bacterial strains was performed by biochemical tests. PCR primers were designed based on the nucleotide sequences of the NHEB/NHEC gene of B. cereus, the hly gene of L. monocytogenes and the C gene of C. jejuni. The specificity of Multiplex PCR method was determined using seven food poisoning bacteria including Salmonella typhi, Shigella dysentery, Yersinia pestis, Staphylococcus aureus, Clostridium perfringens, Clostridium botulinum and Vibrio cholerae. To confirm the reaction, DNA extraction was performed from 30 food samples (milk), and gene amplification was performed by PCR. The length of amplified fragments was 300 bp, 210 bp and 160 bpfor NHEB/NHEC, hly and C genes, respectively. Results: The detection limits of the PCR method were 5, 4 and 3 pg for L. monocytogenes, B. cereus and C. jejuni, respectively. Specifisity test showed that this reaction is spesific to these 3 bacteria. Conclusion: In this study, we introduced a new multiplex PCR method for simultsnus detection of L. monocytogens, B. cereus and C. jejuni. These results can be used for detection of other toxin producing bacteria in food. |
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| نویسندگان مقاله |
علی رضییی | ali razei
applied biotechnology research center, baqiyatallah university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)
رحیم سروری | rahim sorouri
department of microbiology, faculty of medicine, baqiyatallah university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)
سید لطیف موسوی | seyed latif mousavi
department of biology, faculty of basic sciences, shahed university, tehran, iran
سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)
شهرام نظریان | shahram nazarian
biology research centre, faculty of basic science, imam hossein university, tehran, iran
سازمان اصلی تایید شده: دانشگاه جامع امام حسین (Imam hosseyn university)
جعفر امانی | jafar amani
applied microbiology research center, baqiyatallah university of medical science, tehran, iran
حسین آقاملایی | hosien aghamollaei
applied biotechnology research center, baqiyatallah university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_9275.html |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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