Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۱۰، صفحات ۱۱۶۶-۱۱۷۱
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عنوان انگلیسی The effect of the ovarian varicose vein on the DNA methylation in the rat’s oocyte
چکیده انگلیسی مقاله Objective(s): We intended to determine whether the ovarian varicose which is one of the common etiologies of the pelvic congestion syndrome, has the ability to interfere with the DNA methylation reprogramming in the oocyte and thereby affect the oocyte quality or not. Materials and Methods: Varicose model was induced according to the Turner's method in the rats. Briefly, a 20-gauge needle was placed on the left renal vein and a thread was tied over both the needle and the renal vein medial to the insertion of the ovarian vein, and then the needle was removed. Evaluation of prooxidant-antioxidant balance (PAB) was assessed using specific kits and the expression level of the DNA methyltransferase genes Dnmt1, Dnmt3a and Dnmt3L was assessed by Real-time PCR. Immunofluorescent staining for 5-methylcytosine in the oocytes evaluated the global DNA methylation. Results: A significant PAB increase in the ovaries from varicose group was seen. Real-time PCR demonstrated a remarkable decrease in the expression of the Dnmt3a and Dnmt3L which are responsible for de novo DNA methylation in the oocytes. Immunofluorescent staining for 5-mC showed a reduction in the fluorescence intensity in the oocytes collected from the varicose group. Conclusion: Our findings from Real-time PCR and immunocytochemistry suggest that the epigenetic parameters in the oocyte could be affected by varicose induction and these epigenetic alteration has the potential to affect the oocyte quality. We suggest that the epigenetic changes could happen in the oocytes after the induction of ovarian varicose and lead to the oocyte quality reduction or even infertility.
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نویسندگان مقاله امیرحسین محمدی | amirhossein mohammadi
department of anatomy, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

باقر مینایی زنگی | bagher minaei zangi
department of anatomy, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهشید دلفان آذری | mahshid delfan azari
department of radiology, shaheed beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

رفیعه علیزاده | rafieh alizadeh
ent and head amp; neck research center and department, hazrat rasoul akram hospital, iran university of medical sciences iums , tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

محمد صالحی | mohammad salehi
department of biotechnology, shaheed beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

عرفان دانشی | erfan daneshi
department of anatomy, kurdistan university of medical sciences, sanandaj, iran


محمد جعفر رضایی | mohammad jafar rezaei
department of anatomy, kurdistan university of medical sciences, sanandaj, iran


مهدی عباسی | mehdi abbasi
department of anatomy, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_9449.html
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