| چکیده انگلیسی مقاله |
Objective(s): Autologous bone transplantation known as the "gold standard" to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo. Materials and Methods: hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination. In vivo, four cylindrical defects were drilled in the mandibular bones of 5 mongrel dogs and divided randomly into the following groups: I-autologous crushed bone, II- no filling material, III- HA/TCP and PRP, IV- PRP-enriched hAdMSCs seeded on HA/TCP granules. Inserted hAdMSCs were labeled to trace their contribution to bone tissue regeneration. Finally, cell tracing and tissue regeneration were evaluated by immunohistochemistry and histomorphometry methods, respectively. Results: In vitro, co-incubation with HA/TCP granules significantly reduced proliferation and osteogenic differentiation ability of hAdMSCs; while PRP application promoted these capacities (P<0.05). In vivo, PRP-enriched hAdMSCs seeded on HA/TCP granules induced considerable bone formation in osseous defects (P<0.05). It was obviously shown that hAdMSCs were incorporated into the newly-formed bone. Conclusion: Based on this study, application of stem cells could offer a helpful therapeutic tool in bone tissue regeneration. Although inserted hAdMSCs were identifiable throughout the newly-formed bone tissue, their few number could be an indicator of indirect role of hAdMSCs in tissue regeneration. |
| نویسندگان مقاله |
ریحانه شفیعیان | reihaneh shafieian
department of anatomy and cell biology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
مریم مقدم متین | maryam moghaddam matin
stem cell and regenerative medicine research group, academic center for education, culture, and research acecr , khorasan razavi branch, mashhad, iran
امین راه پیما | amin rahpeyma
oral and maxillofacial diseases research center, school of dentistry, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
علیرضا فاضل | alireza fazel
microanatomy research center, school of medicine, mashhad university of medical sciences, mashhad, iran|department of anatomy and cell biology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
حمیده سالاری صدیق | hamideh salari sedigh
department of clinical sciences, faculty of veterinary medicine, ferdowsi university of mashhad, mashhad, iran
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)
آریانه صدر نبوی | ariane sadr nabavi
department of genetics, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
حلیمه حسن زاده | halimeh hassanzadeh
stem cell and regenerative medicine research group, academic center for education, culture, and research acecr , khorasan razavi branch, mashhad, iran
علیرضا ابراهیم زاده بیدسکان | alireza ebrahimzadeh bideskan
microanatomy research center, school of medicine, mashhad university of medical sciences, mashhad, iran|department of anatomy and cell biology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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