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Iranian Journal of Basic Medical Sciences، جلد ۱۴، شماره ۲، صفحات ۱۳۲-۱۳۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Biofilm Formation and Detection of IcaAB Genes in Clinical Isolates of Methicillin Resistant Staphylococcus aureus |
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| چکیده انگلیسی مقاله |
Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less well described in S. aureus. We studied biofilm formation in clinical isolates of MRSA in relation to the presence of the ica operon. Materials and Methods Forty five MRSA were studied for biofilm formation by colony morphology on Congo red agar (CRA) and the microtitre plate assay (MtP). Presence of the ica genes was detected by PCR and specific primers. Results The results showed that 53.3% of the isolates had the potential to form biofilm by colony morphology of which, 75% carried the ica operon. Weak biofilm production was observed in the MtP assay by 57.8%, of which 53.8% harbored the ica operon. However, about 70% of biofilm non-producers also carried the ica operon. Conclusion Overall, there was no agreement between the icaAB gene carriage and biofilm phenotype by either of the two phenotypic methods. However, 91% of biofilm formers on CRA also produced biofilm in the MtP assay. |
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| کلیدواژه‌های انگلیسی مقاله |
Biofilm formation, IcaAB, MRSA, Staphylococcus aureus |
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| نویسندگان مقاله |
فرشته افتخار | fereshteh eftekhar
microbiology department, faculty of biological sciences, shahid beheshti university, g.c., evin, tehran, iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
ترانه دادایی | taraneh dadaei
microbiology department, faculty of biological sciences, shahid beheshti university, g.c., evin, tehran, iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_4978.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45772.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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