Iranian Journal of Basic Medical Sciences، جلد ۱۵، شماره ۶، صفحات ۱۱۵۴-۱۱۶۱

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عنوان انگلیسی Evaluating the Expression of Oct4 as a Prognostic Tumor Marker in Bladder Cancer
چکیده انگلیسی مقاله Objective(s)The key transcriptional regulator Oct4 is one of the self-renewal and differentiation-related factors in cancer stem cells, where it maintains "stemness" state. Cancer stem cells have been identified in a variety of solid malignancies. They are a small population of tumor cells with stem cell characteristics, which are a likely cause of relapse in cancer patients. Due to high incidence, mortality, and recurrence rates of bladder cancer and the necessity of accurate prediction of malignant behavior of the tumors, we evaluated the prognostic value of Oct4 expression in formalin-fixed paraffin-embedded (FFPE) tissues of bladder cancer.Materials and MethodsIn this study, Oct4 expression was evaluated in 52 (FFPE) tissues of bladder cancer. RNA extraction from samples of 30 patients from the archive of Labbafi-Nejad Medical Centre in Tehran was performed and Oct4 expression levels were examined by semi-quantitative RT-PCR. The intracellular distribution of Oct4 protein was also determined by immunohistochemistry (IHC).ResultsThe results revealed a significant correlation between the expression level of Oct4 and the tumors’ grade and stage. A mostly cytoplasmic distribution of Oct4 protein was also confirmed by IHC.ConclusionAll together, our data indicate that the expression level of Oct4 gene is correlated with the clinical and histopathological prognostic indexes of tumors and thus can be considered as a potential prognostic tumor marker.
کلیدواژه‌های انگلیسی مقاله Bladder cancer, Cancer Stem cell, Oct4, prognosis

نویسندگان مقاله نسیم هاتفی | nasim hatefi
department of molecular genetics, faculty of biological sciences, tarbiat modares university, tehran, iran|department of pathology, labbafi-nejad medical centre, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نازیلا nouraee | nazila nouraee
department of molecular genetics, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

محمود پروین | mahmoud parvin
department of pathology, labbafi-nejad medical centre, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

سید امیر محسن ضیایی | seyed amir mohsen ziaee
urology and nephrology research centre, labbafi-nejad medical centre, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

سید جواد مولا | seyed javad mowla
department of molecular genetics, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)


نشانی اینترنتی http://ijbms.mums.ac.ir/article_4934.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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