Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۸، صفحات ۶۲۶-۶۳۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
چکیده انگلیسی مقاله Objective(s):Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value Results: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis. Conclusion: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium
کلیدواژه‌های انگلیسی مقاله Bone marrow, Differentiation, Marine, Osteogenic
نویسندگان مقاله جواد بهارآرا | javad baharara
department of biology amp;amp; research center for animal development applied biology, mashhad branch, islamic azad university, mashhad, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

الهه امینی | elaheh amini
developmental animal biology, faculty of biological sciences, kharazmi university, tehran, iran


محمد امین کراچیان | mohammad amin kerachian
department of medical genetics amp;amp; medical genetic center, faculty of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

مژگان سلطانی | mozhgan soltani
department of biology, research amp;amp; science tehran branch, islamic azad university, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_3198.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45400.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Short Communications
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