Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۹، صفحات ۷۱۰-۷۱۵

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عنوان انگلیسی Effect of thoracic epidural blockade on hypoxia-induced pulmonary arterial hypertension in rats
چکیده انگلیسی مقاله Objective(s): The present study was aimed to investigate the influence of thoracic epidural blockade on hypoxia-induced pulmonary hypertension in rats. Materials and Methods: Forty eight Wistar rats were randomly divided into 4 equal groups, named normoxia hypoxia hypoxia/ ropivacaine and hypoxia/saline. Animals were placed in a hypoxia chamber and instrumented with epidural catheters at the thoracic level. Rats were injected with saline or ropivacaine. Haemodynamic measurements included pulmonary artery pressure and right ventricular hypertrophy. Degree of pulmonary vascular remodeling was determined by Hematoxylin and Eosin (HE) staining. Serum cyclic GMP (cGMP) and TNF-α were measured using radioimmuno assay. Real-time PCR and western boltting were employed to examine the expression of cAMP responding-element binding protein (CREB). Results: We found that the thoracic epidural blockade significantly decreased chronic hypoxia-induced pulmonary hypertension and vascular remodeling in rats. Ropivacaine-treated rats exhibited significantly lower mean pulmonary artery pressure (mPAP), ratio of right ventricular weight to left ventricular plus septal weight (RV/(LV+S)) and wall thickness of pulmonary artery compared with those of control rats. Hypoxia-induced increase in levels of serum cGMP and TNF-α was reversed by thoracic epidural blockade. Moreover, hypoxia increased expression of CREB at mRNA and protein levels which could be suppressed by thoracic epidural blockade. Conclusion:Thoracic epidural blockade reduced mPAP and serum level of TNF-α and increased cGMP. The treatment reversed upregulated expression of CREB at mRNA and protein production.
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نویسندگان مقاله shi huan yu | shi huan yu
institute of pulmonary disease, the first hospital of china medical university, shenyang, china, 110001first hospital of harbin medical university, harbin, china, 150001


جینگ ying chen | jing ying chen
first hospital of harbin medical university, harbin, china, 150001


yi mei زانگ | yi mei zhang
first hospital of harbin medical university, harbin, china, 150001


gui wei jiao | gui wei jiao
first hospital of harbin medical university, harbin, china, 150001


feng qi liu | feng qi liu
first hospital of harbin medical university, harbin, china, 150001


ling fei kong | ling fei kong
first hospital of harbin medical university, harbin, china, 150001



نشانی اینترنتی http://ijbms.mums.ac.ir/article_3335.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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