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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۴، صفحات ۴۰۴-۴۱۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Modulation of IKKβ/NF-κB and TGF-β1/Smad via Fuzheng Huayu recipe involves in prevention of nutritional steatohepatitis and fibrosis in mice |
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| چکیده انگلیسی مقاله |
Objective(s):Fuzheng Huayu recipe (FZHY) exerts significant protective effects against liver fibrosis by strengthening the body’s resistance and removing blood stasis. However, the molecular mechanisms through which FZHY affects liver fibrosis are still unclear. In this study, we examined the expression levels of factors involved in the inhibitor κB kinase-β (IKK-β)/nuclear factor-κB (NF-κB) and transforming growth factor beta 1 (TGF-β1)/Smad signaling pathways to elucidate whether FZHY could attenuate nutritional steatohepatitis and fibrosis in mice. Materials and Methods: C57BL/6J mice were fed with methionine-choline deficient (MCD) diet for 8 weeks to induce fibrotic steatohepatitis. FZHY and/or heme oxygenase-1 (HO-1) chemical inducer (hemin) were administered to mice. The effects of FZHY alone and in combination with hemin were assessed by comparing the severity of hepatic injury, activation of hepatic stellate cells (HSCs), and the expression of oxidative stress, inflammation and fibrogenesis related genes. Results: Administration of FZHY, hemin and FZHY plus hemin significantly ameliorated liver injury. Additionally, our analysis indicated that administration of these agents significantly attenuated oxidative stress, downregulated the expression of pro-inflammatory and pro-fibrotic genes, including IKK-β, NF-κB, monocyte chemoattractant protein-1 (MCP-1), α-smooth muscle actin (α-SMA), TGF-β1, Smad3 and Smad4, and upregulated the expression of the antifibrogenic gene Smad7 (P< 0.001). Conclusion: FZHY-containing therapies prevented nutritional steatohepatitis and fibrosis through modulating the expression of factors associated with the IKKβ/NF-κB and TGF-β1/Smad signaling pathways and oxidative stress related genes. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
rong qi ونگ | rong qi wang
department of traditional and western medical hepatology, third hospital of hebei medical university, shijiazhuang, china
hong mei mi | hong mei mi
department of traditional and western medical hepatology, third hospital of hebei medical university, shijiazhuang, china
هیو li | hui li
department of traditional and western medical hepatology, third hospital of hebei medical university, shijiazhuang, china
su xian zhao | su xian zhao
department of traditional and western medical hepatology, third hospital of hebei medical university, shijiazhuang, china
یان hong جیا | yan hong jia
department of traditional and western medical hepatology, third hospital of hebei medical university, shijiazhuang, china
yue امین nan | yue min nan
department of traditional and western medical hepatology, third hospital of hebei medical university, shijiazhuang, china
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_4291.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45280.pdf |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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