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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۵، صفحات ۴۸۵-۴۹۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Inhibitory effect of corcin on aggregation of 1N/4R human tau protein in vitro |
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| چکیده انگلیسی مقاله |
Objective(s):Alzheimer's disease (AD) is the most common age-related neurodegenerative disorder. One of the hallmarks of AD is an abnormal accumulation of fibril forms of tau protein which is known as a microtubule associated protein. In this regard, inhibition of tau aggregation has been documented to be a potent therapeutic approach in AD and tauopathies. Unfortunately, the available synthetic drugs have modest beneficial efficacy with several side effects. Therefore, pipeline drugs from natural sources with anti-aggregation properties can be useful in the prevention and treatment of AD. Among medicinal plants, saffron (Crocus sativus, L.), as a traditional herbal medicine has different pharmacological properties and can be used as treatment for several nervous system impairment including depression and dementia. Crocin as a major constituent of saffron is the glycosylated form of crocetin. Materials and Methods: In this study, we investigated the inhibitory effect of crocin on aggregation of recombinant human tau protein 1N/4R isoform using biochemical methods and cell culture. Results: Results revealed that tau protein under the fibrillation condition and in the presence of crocin had enough stability with low tendency for aggregation. Crocin inhibited tau aggregation with IC50 of 100 µg/ml. Furthermore, transmission electron microscopy images confirmed that crocin could suppress the formation of tau protein filaments. Conclusion: Inhibitory effect of crocin could be related to its interference with nucleation phase that led to increases in monomer species of tau protein. Based on our results, crocin is recommended as a proper candidate to be used in AD treatment. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
علی علی | ali mohammadi karakani
institute of biochemistry and biophysics, university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)
غلامحسین ریاضی | gholamhossein riazi
institute of biochemistry and biophysics, university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)
سید محمود غفاری | seyed mahmood ghaffari
institute of biochemistry and biophysics, university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)
شاهین احمدیان | shahin ahmadian
institute of biochemistry and biophysics, university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)
فرزاد مختاری | farzad mokhtari
institute of biochemistry and biophysics, university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)
مهشاد جلیلی فیروزی | mahshad jalili firuzi
institute of biochemistry and biophysics, university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)
سیده زهرا بطحایی | seyedeh zahra bathaie
department of clinical biochemistry, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_4412.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45260.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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