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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۵، صفحات ۴۵۹-۴۶۴
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
The Lcn2-engineered HEK-293 cells show senescence under stressful condition |
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| چکیده انگلیسی مقاله |
Objective(s): Lipocalin2 (Lcn2) gene is highly expressed in response to various types of cellular stresses. The precise role of Lcn2 has not been fully understood yet. However, it plays a key role in controlling vital cellular processes such as proliferation, apoptosis and metabolism. Recently it was shown that Lcn2 decreases senescence and increases proliferation of mesenchymal stem cells (MSC) with finite life span under either normal or oxidative stress conditions. However, Lcn2 effects on immortal cell line with infinite proliferation are not defined completely. Materials and Material and Methods: HEK-293 cells were transfected with recombinant pcDNA3.1 containing Lcn2 fragment (pcDNA3.1-Lcn2). Expression of lipocalin2 in transfected cells was evaluated by RT-PCR, real time RT-PCR, and ELISA. Different cell groups were treated with H2O2 and WST-1 assay was performed to determine their proliferation rate. Senescence was studied by β-galactosidase and gimsa staining methods as well as evaluation of the expression of senescence-related genes by real time RT-PCR. Results: Lcn2 increased cell proliferation under normal culture condition, while the proliferation slightly decreased under oxidative stress. This decrease was further found to be attributed to senescence. Conclusion: Our findings indicated that under harmful conditions, Lcn2 gene is responsible for the regulation of cell survival through senescence. |
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| نویسندگان مقاله |
بهاره بهمنی | bahareh bahmani
anatomical sciences research center, kashan university of medical sciences, kashan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)
فاطمه امیری | fatemeh amiri
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
آمنه محمدی روشنده | amaneh mohammadi roushandeh
anatomical sciences department, medicine faculty, hamadan university of medical sciences, hamadan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)
مرضیه بهادری | marzie bahadori
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
مژگان دهقان هراتی | mozhgan dehghan harati
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
مهریار حبیبی رودکنار | mehryar habibi roudkenar
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_4407.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45256.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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