|
|
Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۲، صفحات ۱۷۲-۱۷۷
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa |
|
| چکیده انگلیسی مقاله |
Objective(s): This study was performed to investigate the effects of mucosal acidification on mRNA expression and protein synthesis of cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H2S in gastric mucosa in rats. Materials and Methods:Thirty-two rats were randomly assigned into 4 groups (8 in each), including: the control group, HCl (10 mM) treated group, HCl (100 mM) treated group, and one group to study the effect of Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME). Anesthetized rats underwent tracheostomy and midline laparotomy. Ninety min after the instillation of neutral or acidic solutions, animals were sacrificed and the gastric mucosa was collected to measure the H2S concentration by ELISA method and to quantify mRNA expression of CSE and CBS by quantitative real-time PCR (qRT-PCR). Protein synthesis was also detected by Western blot method. Results:Mucosal acidification with 10 and 100 HCl, significantly increased mucosal levels of H2S (P< 0.01 and P< 0.001) and mRNA (P< 0.01 and P< 0.001) and protein expressions of CSE (P< 0.01 and P< 0.001). L-NAME treatment reversed H2S release to control level. Conclusion:Our findings indicated that mucosal acidification with HCl increased mucosal release of H2S through upregulation of CSE gene and its protein expression. This effect is mainly mediated through the involvement of nitric oxide. |
|
| کلیدواژه‌های انگلیسی مقاله |
Cystathionine gamma lyase H2S, Mucosal acidification, Rat |
|
| نویسندگان مقاله |
سید علی مرد | seyyed ali mard
physiology research center, research center for infectious diseases of digestive system, department of physiology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)
علی ویسی | ali veisi
physiology research center, department of physiology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)
اکرم آهنگرپور | akram ahangarpour
physiology research center, department of physiology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)
محمد کاظم غریب ناصری | mohammad kazem gharib naseri
physiology research center, department of physiology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)
|
|
| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_6541.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45121.pdf |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
Original Article |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|