| چکیده انگلیسی مقاله |
Objective(s):Recently cell therapy is a promising therapeutic modality for many types of disease including acute kidney injury (AKI). Due to the unique biological properties, mesenchymal stem cells (MSCs) are attractive cells in this regard. This study aims to transplant MSCs equipped with nuclear factor E2-related factor 2 (Nrf2) in rat experimental models of acute kidney and evaluate regeneration potential of injured kidney especially expression of injury and repaired biomarkers. Materials and methods:Nrf2 was overexpressed in bone marrow-derived MSCs by pcDNA.3.1 plasmid. AKI was induced using glycerol in rat models. The regenerative potential of Nrf2-overexpressed MSCs was evaluated in AKI-Induced animal models using biochemical and histological methods after transplantation. Expression of repaired genes, AQP1 and CK-18, as well as injury markers, Kim-1 and Cystatin C, was also assayed in engrafted kidney sections. Results:Our results revealed that transplantation of Nrf2-overexpressed MSCs into AKI-induced rats decreased blood urea nitrogen and creatinine and ameliorated kidney regeneration throughout 14 days. Upregulation of repaired markers and downregulation of injury markers were considerable 14 days after transplantation. Conclusions: Overexpression of Nrf2 in MSCs suggests a new strategy to increase efficiency of MSC-based cell therapy in AKI. |
| نویسندگان مقاله |
فاطمه ژاله | fateme zhaleh
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
فاطمه امیری | fatemeh amiri
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
محمد محمدزاده وردین | mohammad mohammadzadeh vardin
department of anatomical sciences and pathology, school of medicine, ardabil university of medical sciences, ardabil, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اردبیل (Ardabil university of medical sciences)
مرضیه بهادری | marzie bahadori
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
میترا دهقان هراتی | mitra dehghan harati
school of medicine, shahid sadoughi university of medical sciences, yazd, iran
مهریار حبیبی رودکنار | mehryar habibi roudkenar
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
ساسان ساکی | sasan saki
department of medical laboratory sciences, faculty of medical sciences, islamic azad university, arak branch, arak, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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