| چکیده انگلیسی مقاله |
Objective(s):Diabetic nephropathy is an important long-term complication of diabetes mellitus which appears to be partially mediated by an increase in secretion of transforming growth factor-β (TGF-β). Fibromodulin, the small leucine-rich proteoglycan, has been proposed to be the potent TGFβ1 modulator. In this study, the therapeutic effects of recombinant adenoviral vectors expressing fibromodulin on TGF-β1 expression on diabetic nephropathy were assessed. Materials and Methods:Forty-eight Sprague-Dawley rats were divided into 4 groups: STZ-induced diabetic rats (diabetic-control), fibromodulin adenovirus vector treated STZ rats (Ad- fibromodulin), and Ad-lacZ-treated STZ rats (Ad-lacZ), and vehicle control (PBS-control). At 10 weeks after STZ treatment, we measured urinary albumin excretion (UAE), urine creatinine was measured by Jaffe method.We also measured kidney TGF-β1 levels by reverse transcription polymerase chain reaction and Real-time PCR. Results:Urine albumin to creatinine ratio or UAE level were listed in four groups. UAE difference between healthy and diabetic rats in all three groups were significant (P≤0.005) and between the control group and treated groups were not significant. Our results indicated that TGF-β1gene expression in diabetic rats were increased and difference between normal group and diabetic group were significant (P≤0.001). Fibromodulin gene transfection mediated by a recombinant adenovirus decreased TGF-β1 level in STZ-induced diabetic rats and TGF-β1 mRNA in diabetic kidney were reduced 2 weeks after Ad-fibromodulin injection. Conclusion:Intraperitoneal injection of adenoviral vectors expressing fibromodulin reduced TGF-β1 level in diabetic rat models. The molecular mechanisms involved in this process require further study. |
| نویسندگان مقاله |
مریم فروتن جزی | maryam foroutan jazi
department of molecular medicine amp;amp; genetics, school of medicine, zanjan university of medical sciences, zanjan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)
علیرضا بیگلری | alireza biglari
cancer gene therapy research center, zanjan university of medical sciences, zanjan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)
سعیده مظلوم زاده | saeideh mazloomzadeh
department of epidemiology, school of medicine, zanjan university of medical sciences, zanjan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)
پاوول kingston | paul kingston
vascular gene therapy unit, research school of clinical amp;amp; laboratory sciences, manchester academic health science center, the university of manchester, manchester, uk
علی رمضانی | ali ramazani
cancer gene therapy research center, zanjan university of medical sciences, zanjan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)
جواد توکلی بزاز | javad tavkoli bazzaz
department of genetics, school of medicine, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مهدی اسکندری | mehdi eskandari
department of physiology, school of medicine, zanjan university of medical sciences, zanjan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)
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