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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۴، صفحات ۴۰۲-۴۱۰
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| عنوان انگلیسی |
Effect of carvacrol on various cytokines genes expression in splenocytes of asthmatic mice |
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| چکیده انگلیسی مقاله |
Objective(s):With regard to pharmacological effects of carvacrol on the respiratory system, its effect on cytokines genes expression in splenocytes of asthmatic mice was examined in this study. Materials and Methods:Splenocytes were isolated from non-sensitized (control group), sensitized mice to ovalbumin (OVA) (group S), and S animals treated with dexamethasone, and three concentrations of carvacrol. IL-4, IFN-γ, TGF-β, FOXP3, and IL-17 genes expression were carried out in cultured splenocytes using the real-time PCR method. Results:Compared to the control group, IFN-γ and FOXP3 genes expression were significantly decreased (P< 0.001 for both cases), but IL-4 and IL-17 genes expression were significantly increased in the S group (P< 0.001 and P< 0.05, respectively). IL-4 gene expression due to treatment of all concentrations of carvacrol, TGF-β gene expression due to its two higher concentrations, and IL-17 gene expression due to its high concentration were significantly decreased compared to group S (P< 0.01 to P< 0.001). IFN-γ gene expression was significantly increased due to last carvacrol concentration (300 µg/ml, P< 0.01), and FOXP3 due to its two last concentrations (150 and 300 µg/ml, P< 0.05 and P< 0.001, respectively) in treated S splenocytes. Dexamethasone treatment of sensitized splenocytes only showed significant inhibitory effect on IL-4 and TGF-β genes expression (P< 0.001 for both cases). Conclusion: These results showed the immunomodulatory effect of carvacrol indicating increased IFN-γ and FOXP3 but decreased IL-4, TGF-β, and IL-17 genes expression, which was more selective than the effect of dexamethasone in sensitized mice splenocytes, which indicates its possible therapeutic value in allergy, autoimmunity, and infectious diseases. |
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| نویسندگان مقاله |
مجید کیان مهر | majid kianmehr
neurogenic inflammation research center and department of physiology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
عبدالرحیم رضایی | abdolrahim rezaei
inflammation and inflammatory diseases research center, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
محمد حسین بسکابادی | mohammad hossein boskabady
neurogenic inflammation research center and department of physiology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_6812.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45091.pdf |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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