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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۹، صفحات ۹۴۰-۹۴۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine |
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| چکیده انگلیسی مقاله |
Objective(s): Endometriosis is a complex gynecologic disease with unknown etiology. Noscapine has been introduced as a cancer cell suppressor. Endometriosis was considered as a cancer like disorder, The aim of present study was to investigate noscapine apoptotic effect on human endometriotic epithelial and stromal cells in vitro. Materials and Methods:In this in vitro study, endometrial biopsies from endometriosis patients (n=9) were prepared and digested by an enzymatic method (collagenase I, 2 mg/ml). Stromal and epithelial cells were separated by sequential filtration through a cell strainer and ficoll layering. The cells of each sample were divided into five groups: control (0), 10, 25, 50 and 100 micromole/liter (µM) concentration of noscapine and were cultured for three different periods of times; 24, 48 and 72 hr. Cell viability was assessed by colorimetric assay. Nitric oxide (NO) concentration was measured by Griess reagent. Cell death was analyzed by Acridine Orange (AO)–Ethidium Bromide (EB) double staining and Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. Data were analyzed by one-way ANOVA. Results: Viability of endometrial epithelial and stromal cells significantly decreased in 10, 25, 50 and 100 µM noscapine concentration in 24, 48, 72 hr (P<0.05) and apoptotic index increased in 25, 50 and 100 µM noscapine concentrations in 48 hr significantly (P< 0.05). Concentrations of NO didn’t show a significant decrease. Conclusion: Noscapine increased endometriotic epithelial and stromal cell death and can be suggested as a treatment for endometriosis. |
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| کلیدواژه‌های انگلیسی مقاله |
Apoptosis, Endometriosis, Nitric Oxide, Noscapine |
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| نویسندگان مقاله |
محمد رسول خزایی | mohammad rasoul khazaei
fertility and infertility research center, kermanshah university of medical sciences, kermanshah, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)
زهرا رشیدی | zahra rashidi
fertility and infertility research center, kermanshah university of medical sciences, kermanshah, iran|department of anatomy, medical faculty, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)
فرزانه چوبساز | farzaneh chobsaz
fertility and infertility research center, kermanshah university of medical sciences, kermanshah, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)
مظفر خزایی | mozafar khazaei
fertility and infertility research center, kermanshah university of medical sciences, kermanshah, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_7593.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-45012.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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