Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۱۱، صفحات ۱۱۸۶-۱۱۹۲

عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells
چکیده انگلیسی مقاله Objective(s): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. Materials and Methods: BMMSCs were collected from the bone marrow of 6-8-week old NMRI mice and their mesenchymal entities were proven using superficial markers (expression of CD44 and CD73 and non-expresion of CD45 and CD11b) by fow cytometry. Their multi-potential entities were proved with differentiation to osteogenic and adipogenic cells for 21 days. Also isolated Sertoli cells were enriched using lectin coated plates and Sertoli cell condition medium (SCCM) was collected. Sertoli cells were identified by immunocytochemistry and Vimentin marker. The cells were then differentiated into germ cells with SCCM for 2 weeks. Finally induced cells were evaluated by RT-PCR and immunocytochemistry. Results: Differentiation of mesenchymal stem cells to osteoblast and adipocyte showed their multi-potential property. Expression of CD44 and CD73 and non-expression of CD45 and CD11b confirmed mesenchyme cells. Immunocytochemistry and RT-PCR results showed expression of germ cells specific marker (Mvh). Conclusion: This study confirmed the effect of SCCM as a motivational factor that can used for differentiation of germ cells from BMMSCs.
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نویسندگان مقاله مهدیه حاجیان منفرد | mahdieh hajian monfared
department of anatomical sciences, school of medicine, tehran university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

باقر مینایی | bagher minaee
department of anatomical sciences, school of medicine, tehran university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

طیبه رستگار | tayebeh rastegar
department of anatomical sciences, school of medicine, tehran university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

ابراهیم khrazinejad | ebrahim khrazinejad
department of anatomical sciences, school of medicine, tehran university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمد بربرستانی | mohammad barbarestani
department of anatomical sciences, school of medicine, tehran university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)


نشانی اینترنتی http://ijbms.mums.ac.ir/article_7818.html
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کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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