فرآیند و کارکرد گیاهی، جلد ۵، شماره ۱۸، صفحات ۳۵-۴۲
عنوان فارسی Effect of water deficit on physiological response, total protein and gene expression of Rab۱۷ in wheat (Triticumaestivum)
چکیده فارسی مقاله Drought is one of the most adverse environmental constraints that impact on plant growth and reduce crop yields.To discus effect of water deficit on molecular and physiological responses, two cultivars of bread wheat (Sardari and Zarin) were selected (based on results accumulated during three years of research described in Heidari et al (1381). The plantswere cultivated in a completely randomized design with three replication in plastic pots containing field soil in growth chambers (30/ 20 °C, 14 h day/ 10 night, 60% R.H).Their response to water stress was performed at the physiological level by determination of relative water content (RWC), chlorophyll, free proline and total proteins content for duration of the drought treatment and subsequent re-watering.All physiological parameters were affected by drought stress. Relative water content of zarin cultivar reduced significantly. The content of chlorophyll a and total chlorophyll in the both cultivars decrease significantly but the change of the content of chlorophyll b in two cultivars wasn't significant. The free proline contents of two cultivars increased significantly and both cultivars could recover to normal condition by re-watering after short term drought. SDS-PAGE electrophoresis of leaf proteins under control and drought treatments showed some up and down regulated proteins.Modification in the expression level of dehydrin (DHN) gene (Rab17) was also analyzed by reverse transcription–polymerase chain reaction (RT-PCR). This gene was expressed slightly in the well water plants of two cultivars, but the gene expression of sardari cultivar increased significantly after long term drought.
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عنوان انگلیسی Effect of water deficit on physiological response, total protein and gene expression of Rab17 in wheat (Triticumaestivum)
چکیده انگلیسی مقاله Drought is the cause of adverse environmental impacts on plant growth and crop yield. In this study, the effects of water deficit on plant molecular and physiological responses were investigated using two cultivars (namely, Sardari and Zarin) of bread wheat selected based on the results of a three-year research. For the purposes of this study, they were grown in plastic pots containing field soil and maintained in growth chambers (30/20 °C, 14/10 h day/night, 60% R.H). The cultivars had been The experiment was conducted in a completely randomized design with three replications. Plant response to water stress was evaluated at the physiological level by determining the relative water content (RWC) as well as chlorophyll, free proline, and total protein contents following the drought treatment with subsequent re-irrigation. All the physiological parameters were found to be affected by drought stress. The Zarin cultivar exhibited a significant decrease in its RWC. Chlorophyll a and total chlorophyll in both the cultivars showed significant decreases but chlorophyll b did not exhibit any significant variation in either cultivar. The free proline content increased significantly in both cultivars such that they were both restored their normal proline contents life when irrigation was resumed after a short-term experimental drought stress. SDS-PAGE electrophoresis of leaf proteins in both control and experimental samples revealed regulating adjustments in protein contents. Modification in the expression level of the dehydrin (DHN) gene (that is, Rab17) was also analyzed by reverse transcription–polymerase chain reaction (RT-PCR). This gene was expressed slightly in the well watered plants of the two cultivars, but the gene expression in the Sardari cultivar increased significantly after the long-term experimental drought.
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نویسندگان مقاله سدابه جهانبخش گدهکهریز | soodabe jahanbakhsh godekahriz
agronomy and plant breeding department, university of mohaghegh ardabili
دانشگاه محقق اردبیلی
سازمان اصلی تایید شده: دانشگاه محقق اردبیلی (Mohaghegh ardabili university)

حمیده چیلان | hamideh chilan
agronomy and plant breeding department, university of mohaghegh ardabili
محقق

خدیجه رضایی | khadijeh razavi
genetic engineering and biotechnology, tehran, iran
مرکز تحقیقات ملی مهندسی ژنتیک و بیوتکنولوژی، تهران، ایران.

نشانی اینترنتی http://jispp.iut.ac.ir/browse.php?a_code=A-10-405-6&slc_lang=en&sid=fa
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کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده تنش خشکی
نوع مقاله منتشر شده پژوهشی
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