| چکیده انگلیسی مقاله |
Background & Aims: Clostridium difficile has been identified as a pathogen in antibiotic associated diarrhea (AAD), pseudomembranous colitis and also nosocomial diarrhea. The present study was performed to find the prevalence of toxigenic strains of C .difficile isolated from diarrhea patients hospitalized in Tehran hospitals. Method: A total of 98 fecal samples obtained during July to December 2010 were studied. Samples were rapidly cultured on the CCFA medium and incubated at the anaerobic conditions. Then ELISA was done to detect toxin A and B in the stool. Molecular identification of C.difficile was done by cdd3 universal primer and toxin A gene (tcdA), toxin B gene (tcdB) and binary toxin profiles were determined by PCR method. Results: from a total of 98 fecal samples, 15 samples (15.3%) were positive of which, 12 strains (21.2%) were A+B+, 2 strains (2%) were A+B-, and 1 strain (1%) was A-B+. Conclusion: This study showed that Clostridium difficile is an important pathogen in the development of nosocomial diarrhea. Therefore, routine detection of C.difficile in suspected cases is recommended. |
| نویسندگان مقاله |
o عزیزی |
master of medical microbiology, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
محمد مهدی اصلانی | m m
professor, department of microbiology, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
m عظیمی راد | azimi rad
bachelor of laboratory science, research center of gastroenterology and liver disease, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
m آل بویه |
assistant professor of microbiology, research center of gastroenterology and liver disease, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
سیده فاطمه موسوی | s f
assistant professor, department of microbiology, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
محمدرضا زالی | m r
professor, research center of gastroenterology and liver disease, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
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