| چکیده انگلیسی مقاله |
Background & Aims: Induced pluripotent stem cells (iPSCs) have the capability to undergo unlimited selfrenewal and differentiation into all cell types in the body. These cells are artificially derived from a nonpluripotent cell, typically human dermal fibroblasts (HDFs). The study of cytogenetic stability of these cells, in order to use iPS cells and apply studies in therapeutic applications, is essential. Methods: In the present experimental study, HDFs were isolated and cultured from human foreskin samples. The cytogenetic stability of these cells was evaluated in early passages (1-3) of HDFs using karyotype test and alkaline comet assay technique. The HDF cells treatment with hydrogen peroxide (H2O2) was used as a positive control for alkaline comet assay. The iPS cells with low passage (4-7) derived from reprogrammed HDFs were cultured on mouse embryonic fibroblast (MEF) feeder layer and cytogenetic stability of these cells were evaluated in early passages using karyotype test and alkaline comet assay technique. Results: The iPS cells in early passages (4-7) had normal karyotype (46, XY) and DNA damage and comet were not observed in these cells. In addition, HDF cells showed normal karyotype in early passages (1-3), but using comet assay, abnormality and DNA damages were observed in positive control (HDFs treated with H2O2). The comparison of alkaline comet assay parameters of iPS and HDF cells with positive control group showed statistically significant differences (P < 0.05). Conclusion: Since the comet assay is a sensitive technique for finding DNA damage, it is best if cytogenetic stability of these cells were evaluated before performing functional experiments on iPS cells. Therefore, for the precise evaluation of DNA damage and cytogenetic stability of iPS cells, the two techniques could complement each other |
| نویسندگان مقاله |
پیام قاسمی دهکردی | ghasemi dehkordi
research assistant, cellular and molecular research center, school of medicine, shahrekord university of medical sciences, shahrekord, iran
مهدی اله بخشیان فارسانی | allahbakhshian farsani
associate professor, department of hematology and blood banking, school of allied medicine, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
نرگس عبدیان |
research assistant, cellular and molecular research center, school of medicine, shahrekord university of medical sciences, shahrekord, iran
حمیده جعفری قهفرخی | jafari ghahfarokhi
research assistant, cellular and molecular research center, school of medicine, shahrekord university of medical sciences, shahrekord, iran
جواد صفاری چالشتری | saffari chaleshtori
research assistant, clinical biochemistry research center, school of medicine, shahrekord university of medical sciences, sharekord, iran
مرضیه صادقیانی |
research assistant, cellular and molecular research center, school of medicine, shahrekord university of medical sciences, shahrekord, iran
امین میرزاییان |
research assistant, cellular and molecular research center, school of medicine, shahrekord university of medical sciences, shahrekord, iran
مرتضی هاشم زاده چالشتری | hashemzadeh chaleshtori
professor, cellular and molecular research center, school of medicine, shahrekord university of medical sciences, shahrekord, iran
|