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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۲، صفحات ۲۰۹-۲۱۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients |
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| چکیده انگلیسی مقاله |
Objective(s): High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes. Materials and Methods: Having prepared dental pulp tissues of pulpitis and healthy controls, macrophage were isolated and cultured. Macrophages were thereafter stimulated by HMGB1 time course. RT-QPCR, flowcytometer, immunofluorescence, Western blotting, and ELISA techniques were used in the present research. Results: Our results showed that the expression of TLR2 and TLR4 on macrophages stimulated with HMGB1 increased in pulpitis compared with controls (macrophages without HMGB1 stimulation) with a statistical significance (P<0.001). In addition, the levels of IL-17, IL-23, and IL-6 in supernatants from cultured macrophages stimulated with HMGB1 from pulpitis increased, and NF-kB, the downstream target of TLR2 and TLR4, also showed a marked elevation after macrophages’ stimulation by HMGB1. Conclusion: The evidence from the present study suggests that the enhanced TLR2 and TLR4 pathways and Th17 cell polarization may be due to HMGB1 stimulation in pulpitis. |
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| کلیدواژه‌های انگلیسی مقاله |
HMGB1, Pulpitis, TLR2, TLR4 |
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| نویسندگان مقاله |
جواد محمودی | javad mahmoudi
neurosciences research center, tabriz university of medical science, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
بابک sabermarouf | babak sabermarouf
neurosciences research center, tabriz university of medical science, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
بهزاد برادران | behzad baradaran
immunology research center, tabriz university of medical science, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
لیلا سادات حاتم نژاد | leila sadat hatamnezhad
immunology research center, tabriz university of medical science, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)
سیامک صندوقچیان | siamak sandoghchian shotorbani
department of immunology, tabriz branch, islamic azad university, tabriz, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_8250.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-321249.pdf |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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