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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۲، صفحات ۱۷۸-۱۸۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Expression pattern of neurotrophins and their receptors during neuronal differentiation of adipose-derived stem cells in simulated microgravity condition |
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| چکیده انگلیسی مقاله |
Objective(s): Studies have confirmed that microgravity, as a mechanical factor, influences both differentiation and function of mesenchymal stem cells. Here we investigated the effects of simulated microgravity on neural differentiation of human adipose-derived stem cells (ADSCs). Materials and Methods:We have used a fast rotating clinostat (clinorotation) to simulate microgravity condition. Real-time PCR and flow cytometry analysis were used to evaluate the regulation of neurotrophins, their receptors, and neural markers by simulated microgravity and their impact on neural differentiation of cells. Results: Our data revealed that simulation microgravity up-regulated the expression of MAP-2, BDNF, TrkB, NT-3, and TrkC both before and after neural differentiation. Also, the neural cells derived from ADSCs in microgravity condition expressed more MAP-2, GFAP, and synaptophysin protein in comparison to the 1G control. Conclusion: We showed that simulated microgravity can enhance the differentiation of mesenchymal stem cells into neurons. Our findings provide a new strategy for differentiation of ADSCs to neural-like cells and probably other cell lineages. Meanwhile, microgravity simulation had no adverse effects on the viability of the cells and could be used as a new environment to successfully manipulate cells. |
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| کلیدواژه‌های انگلیسی مقاله |
ADSCs, Microgravity, Neural differentiation Neurotrophin |
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| نویسندگان مقاله |
وجیهه زرین پور | vajiheh zarrinpour
department of biology, fars science and research branch, islamic azad university, marvdasht, iran|department of biology, marvdasht branch, islamic azad university, marvdasht, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
زهرا حاج ابراهیمی | zahra hajebrahimi
aerospace research institute, ministry of science research and technology, tehran, iran
سازمان اصلی تایید شده: وزارت علوم تحقیقات و فناوری (Ministry of science research and technology)
مجتبی جعفری نیا | mojtaba jafarinia
department of biology, fars science and research branch, islamic azad university, marvdasht, iran|department of biology, marvdasht branch, islamic azad university, marvdasht, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_8244.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-321245.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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