| چکیده انگلیسی مقاله |
Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of mouse IgG2a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-10: Fcγ2a and pPICZαA-CFP-10:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-12, IL-4, IL-17 and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-12 in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-10:Fcγ2a and CFP-10:His) could excite good response in Th1-cells. The Fc-tagged protein had a stronger Th1 response with low levels of IL-4, as compared to CFP-10:His. However, the highest level of Th1 response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-10: Fcγ2a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-10 protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen. |
| نویسندگان مقاله |
علی اصغر باغانی | ali asghar baghani
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
سامان سلیمانپور | saman soleimanpour
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
هادی فارسیانی | hadi farsiani
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
آرمان مساوات | arman mosavat
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
مسعود یوسفی | masoud yousefi
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
زهرا مشکات | zahra meshkat
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
سید عبدالرحیم رضایی | seyed abdolrahim rezaee
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|inflammation and inflammatory diseases research centre, medical school, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
سعید عامل جامه دار | saeid amel jamehdar
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
محمدرضا اکبری عیدگاهی | mohammad reza akbari eydgahi
biotechnology research center, semnan university of medical sciences, semnan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی سمنان (Semnan university of medical sciences)
حمید صادقیان | hamid sadeghian
organic chemistry, department of laboratory sciences, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
کیارش قزوینی | kiarash ghazvini
antimicrobial resistance research center, bu-ali research institute, mashhad university of medical sciences, mashhad, iran|department of microbiology and virology, school of medicine, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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