| چکیده انگلیسی مقاله |
Abstract
Background: Control of mosquito populations is, essential for disease control; the widespread use of chemical insecticides has led to mosquitoes developing resistance to such treatments and has polluted the ecosystem. Larvicidal agents can be produced using microbial resources, which are environmentally friendly, biodegradable, and economical. The study's goal was to evaluate the larvicidal activity of metabolites isolated from Nocardia and Streptomyces species against Anopheles stephensi.
Methods: Four metabolites isolated from Nocardia and Streptomyces strains were exanimated for larvicidal activity. The experiments were performed for 24, 48, and 72 hours at room temperature (28±2 ºC). 300, 350, 400, 450, 500, 550, and 600 µl of Actinobacteria metabolites were added to 100 cc of dechlorinated water. Fourth-stage larvae were placed in dechlorinated water as a control. LC50 and LC90 were calculated using toxicity data and analyzed.
Results: All metabolites had a statistically significant influence on mosquito larvae (p< 0.05). At 24, 48, and 72 hours, the LC50 for N2 (Nocardia2) was 417, 386, and 370, respectively, and, the LC90 was 650, 595, and 561. Moreover, LC50 for N4 (Nocardia4) was 389, 376, and 347 and LC90 were 591, 565, and 533 and LC50 for N5 (Nocardia5) was 390, 357, and 341 and LC90 were 589, 532, and 484. In addition, LC50 for S921 (Streptomyces 921) was 484, 416, and 382, and LC90 was 701, 612, and 574.
Conclusions: Four isolates have demonstrated a substantial larval mortality rate against Anopheles stephensi. This is an effective technique for controlling Anopheles stephensi that has no detrimental environmental impact. |
| نویسندگان مقاله |
| Marjan Seratnahaei
North Tehran Branch, Islamic Azad University, Tehran, Iran.
| Alireza Zahraei-Ramazani
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| Seyyed Saeed Eshraghi
School of Public Health, Tehran University of Medical Science, Tehran, Iran.
| Mehdi Yaseri
Tehran University of Medical Sciences
| Parviz Pakzad
faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
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