Journal of Arthropod-Borne Diseases، جلد ۱۷، شماره ۲، صفحات ۱۶۲۳-۱۶۲۳

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عنوان انگلیسی Metagenomics Characterization of Ixodes ricinus Intestinal Microbiota as Ma-jor Vector of Tick-Borne Diseases in Domestic Animals
چکیده انگلیسی مقاله Background: Understanding the microbiota of disease vectors can help for developing new strategies to prevent the transmis­sion of vector pathogens. Ixodes ricinus is one of the most notorious tick vectors with increasing importance in Iran and other parts of the world while there is limited data on its microbiota. This study aimed to use metagenomics for identifying the I. ricinus tick’s microbiota of Iran. Methods: A total of 39 adult ticks were collected from Mazandaran (21 females), Gilan (17 females), and Golestan (1 male). Five tick pools prepared from 39 adults of I. ricinus were subjected to metagenomics analysis. The data were analyzed by targeting the V6 region of the 16S rRNA gene by Illumina 4000 Hiseq sequencing. Results: Among hundreds of intestinal microbiota identified by metagenomics, various pathogenic microorganisms distributed in 30 genera and species including those responsible for tick-borne diseases resided in the genera Coxiella, Rickettsia, and Burkholderia were found. Conclusion: Our results indicated that metagenomics identifies bacteria genera and species which cannot be easily rec­ognized by routine methods. The presence of such pathogenic bacteria indicates the importance of possible zoonotic diseases in this region which could affect public health. These results further substantiate the importance of advanced metagenomics analyses to identify neglected tick-borne pathogens which enable researchers to provide efficient mapping roads for the management of emerging and re-emerging infectious diseases.
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نویسندگان مقاله | Manijeh Yousefi Behzadi
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infec-tious Diseases, Pasteur Institute of Iran, Tehran, Iran, National Reference Laboratory of Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar-Ahang, Hamadan, Iran


| Atefeh Mehrabi
School of Advanced Medical Sciences, Islamic Azad University, Tehran Medical Branch, Tehran, Iran


| Mozhgan Ahmadinezhad
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infec-tious Diseases, Pasteur Institute of Iran, Tehran, Iran


| Mahdi Rohani
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran


| Saeid Reza Naddaf
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran


| Amin Bagheri
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research, Tehran, Iran


| Masoomeh Shams-Ghahfarokhi
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Ebrahim Maghsoudlou
Department of Computer Science, School of Computing, Southern Illinois University Carbondale, Illinois


| Nooshin Mojahed
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale, Illinois


| Leila Mounesan
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran


| Zahra Tahmasebi
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran, National Reference Laboratory of Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar-Ahang, Hamadan, Iran


| Aria Sohrabi
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran, National Reference Laboratory of Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar-Ahang, Hamadan, Iran


| Mostafa Salehi-Vasiri
Department of Arboviruses and Viral Hemorrhagic Fevers, Pasteur Institute of Iran, Tehran, Iran


| Zahra Salehi
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran


| Mehdi Razzaghi-Abyaneh
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran



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