| چکیده انگلیسی مقاله |
Background and purpose: Ferula gummosa ( F. gummosa ), a potent medicinal herb, has been shown to possess anticancer activities in vitro . The present examination evaluated the cytotoxic and apoptogenic impacts of F. gummosa gum on the U87 glioblastoma cells. Experimental approach: MTT assay to determine the cell viability, flow cytometry by annexin V/FITC-PI to apoptosis evaluation, reactive oxygen species (ROS) assay, and quantitative RT-PCR were performed. Findings / Results: The results revealed that F. gummosa inhibited the growth of U87 cells in a concentration- and time-dependent manner with IC 50 values of 115, 82, and 52 μg/mL obtained for 24, 48, and 72 h post-treatment, respectively. It was also identified that ROS levels significantly decreased following 4, 12, and 24 h after treatment. The outcomes of flow cytometry analysis suggested that F. gummosa induced a sub-G1 peak which translated to apoptosis in a concentration-dependent manner. Further examination revealed that F. gummosa upregulated Bax/Bcl-2 ratio and p53 genes at mRNA levels. Conclusion and implications: Collectively, these findings indicate that sub-G1 apoptosis and its related genes may participate in the cytotoxicity of F. gummosa gum in U87 cells. |
| نویسندگان مقاله |
| Amir R. Afshari
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
| Seyed Hadi Mousavi
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
| Golrokh Mousavi
Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, I.R. Iran.
| Sanam Daneshpour Moghadam
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, I.R. Iran.
| Abolfazl Maghrouni
Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad,
| Hossein Javid
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
| Zahra Tayarani-Najaran
Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, I.R. Iran.
| Bahram Bibak
Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, I.R. Iran.
| Hamid Mollazadeh
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
| Azar Hosseini
|