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International Journal of Reproductive BioMedicine، جلد ۲۰، شماره ۷، صفحات ۵۶۱-۵۶۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium |
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| چکیده انگلیسی مقاله |
Background: Preimplantation genetic diagnosis (PGD) has been used as an option for couples with the possibility of having a baby with a genetic disorder. The common method for performing this test involves isolating one cell from day 3 or a few cells from day 5 embryos and performing genetic studies on the cell-extracted DNA. This method is invasive and can cause abortion after implantation in the uterus. Because of this, 2 noninvasive methods for performing a PGD have been studied :PGD using blastocyst fluid and PGD using embryo culture medium. Objective: The aim of this study is to determine the sensitivity of polymerase chain reaction (PCR) technique to detect Y chromosome using cell-free DNA within culture medium for gender prediction of blastocysts. Materials and Methods: In this study, the gender of 30 embryos on day 5 was determined using embryonic DNA extraction from the culture medium, and the PCR technique to evaluate genes sex-determining Region Y and fragile X mental retardation. Then, their accuracy was assessed using ultrasound. Results: The results of the PCR technique showed that 7 embryos were male, but an ultrasound revealed that 13 were male. Conclusion: The given results indicated that because of the low amount of DNA extracted from the culture medium, the diagnosis of the existence of the Y chromosome by this method is still not accurate enough for detecting the gender of the embryo. |
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| کلیدواژه‌های انگلیسی مقاله |
Preimplantation diagnosis, Embryo implantation, Culture media, Blastocyst, Polymerase chain reaction. |
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| نویسندگان مقاله |
| Somayeh Barzanouni
Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
| Farideh Moramezi
Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
| Mahvash Zargar
Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
| Hamid Galehdari
Department of Genetics, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
| Masoud Hemadi
Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
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| نشانی اینترنتی |
http://ijrm.ssu.ac.ir/browse.php?a_code=A-10-1605-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Perinatology |
| نوع مقاله منتشر شده |
Original Article |
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