| چکیده انگلیسی مقاله |
Objective(s): The current study’s objectives were to obtain different extracts and essential oils of Symphytum kurdicum and Symphytum asperrimum and to determine the chemical composition, as well as to evaluate free radical scavenging activity (IC50) and minimum bactericidal concentration (MBC), and the effect of liposomal formulation on antimicrobial properties.Materials and Methods: Air-dried powdered aerial parts of S. kurdicum and S. asperrimum were used. The antioxidant and antibacterial properties, essential oil compositions, total phenol, and flavonoid contents of different fractions were determined by DPPH test, disk diffusion assay, gas chromatography-mass spectrometry, Folin-ciocalteu reagent, and colorimetric assay method, respectively. The film hydration method was used to fabricate nanoparticles.Results: GC-MS analysis indicated that hexafarnesyl acetone was a major essential oil component. n-butanol and ethyl acetate extracts of S. kurdicum had the highest anti-oxidant activity. Extracts of both plants showed antimicrobial activity. The extracts’ maximum inhibition zones against Staphylococcus epidermidis were established. A particle size analyzer detected the formulation size of 140 nm. The optimum formulation of liposomes contains the ratio of 75 mg lecithin, 25 mg cholesterol, and 50 mg herbal extract. Despite the nanoparticles’ appropriate particle size, the liposomal extract’s antimicrobial effect was lower than that of the free form.Conclusion: Our findings demonstrated that extracts have significant antibacterial and anti-oxidant activities, attributed to their bioactive constituents. |
| کلیدواژههای انگلیسی مقاله |
Anti-Oxidants, Boraginaceae, Comfrey, Flavonoids, Liposome, Microbial sensitivity tests, Nanoparticle |
| نویسندگان مقاله |
| Elaheh Mahmoudzadeh
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
| Hossein Nazemiyeh
Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Research Centre for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
| Hadi Valizadeh
Research Center for Pharmaceutical Nanotechnology, and Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
| Farnaz Khaleseh
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Pharmaceutical Sciences Research Center, Health Institute and School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
| Samin Mohammadi
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
| Sanaz Hamedeyazdan
Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Research Center for Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran
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