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Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۱۱، صفحات ۱۴۰۹-۱۴۱۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
In silico analysis and expression of a new chimeric antigen as a vaccine candidate against cutaneous leishmaniasis |
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| چکیده انگلیسی مقاله |
Objective(s): Since leishmaniasis is one of the health problems in many countries, the development of preventive vaccines against it is a top priority. Peptide vaccines may be a new way to fight the Leishmania infection. In this study, a silicon method was used to predict and analyze B and T cells to produce a vaccine against cutaneous leishmaniasis.Materials and Methods: Immunodominant epitope of Leishmania were selected from four TSA, LPG3, GP63, and Lmsti1 antigens and linked together using a flexible linker (SAPGTP). The antigenic and allergenic features, 2D and 3D structures, and physicochemical features of a chimeric protein were predicted. Finally, through bioinformatics methods, the mRNA structure was predicted and was produced chemically and cloned into the pLEXY-neo2 vector.Results: Results indicated, polytope had no allergenic properties, but its antigenicity was estimated to be 0.92%. The amino acids numbers, molecular weight as well as negative and positive charge residuals were estimated 390, ~41KDa, 41, and 30, respectively. The results showed that the designed polytope has 50 post-translationally modified sites. Also, the secondary structure of the protein is composed of 25.38% alpha-helix, 12.31% extended strand, and 62.31% random coil. The results of SDS-PAGE and Western blotting revealed the recombinant protein with ̴ 41 kDa. The results of Ramachandran plot showed that 96%, 2.7%, and 1.3% of amino acid residues were located in the preferred, permitted, and outlier areas, respectively. Conclusion: It is expected that the TLGL polytope will produce a cellular immune response. Therefore, the polytope could be a good candidate for an anti-leishmanial vaccine. |
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| کلیدواژه‌های انگلیسی مقاله |
Bioinformatics, Leishmania major, Polytope, TLGL, Vaccine |
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| نویسندگان مقاله |
| Leila Motamedpour
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
| Abdolhossein Dalimi
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
| Majid Pirestani
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
| Fatemeh Ghafarifar
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
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| نشانی اینترنتی |
https://ijbms.mums.ac.ir/article_16432.html |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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