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Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۱۲، صفحات ۱۵۷۶-۱۵۸۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Curcumin inhibits APOE4-induced injury by activating peroxisome proliferator-activated receptor-γ (PPARγ) in SH-SY5Y cells |
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| چکیده انگلیسی مقاله |
Objective(s): The human apolipoprotein E4 (APOE4) is associated with various brain injuries and neurodegenerative changes. Curcumin is an active ingredient isolated from the root of turmeric and is believed to have therapeutic effects on neurodegenerative diseases. The aim of this study was to investigate the effects of curcumin on APOE4-induced neurological damage and explore its molecular mechanisms.Materials and Methods: SH-SY5Y cells were pretreated with curcumin for 24 hr and transfected with human APOE4 gene using Lipofectamine 2000. Then, the effect of curcumin on the transfected cells was detected by ELISA, immunofluorescence staining and Western blot.Results: The production or expression of proinflammatory cytokines and proteins, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was significantly increased in SH-SY5Y cells transfected with APOE4, and curcumin inhibited APOE4-induced cellular inflammatory damage. Western blot analysis showed that, after transfection with APOE4, the expression of total nuclear factor kappa B (NF-κB) p65 and p-NF-κB p65 in the nucleus was increased, and curcumin inhibited the nuclear translocation of p65. The overexpression of APOE4 inhibited the expression of peroxisome proliferator-activated receptor-γ (PPARγ), whereas curcumin reversed and increased the expression of PPARγ protein. Down-regulating PPAR-γ with the inhibitor GW9662 and the shPPARγ gene confirmed that the NF-κB signaling pathway was inhibited by PPARγ. Conclusion: This study suggests that APOE4 overexpression can induce cellular inflammatory damage, and pretreatment of curcumin could exert an anti-inflammatory effect by upregulating the expression of PPARγ to inhibit the activation of NF-κB signaling pathway. |
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| کلیدواژه‌های انگلیسی مقاله |
Apolipoprotein E4 Curcumin Neuroinflammation NF, KappaB PPAR gamma |
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| نویسندگان مقاله |
| Minghui Wang
Pharmaceutical Institute, Pharmaceutical College of Henan University, Kaifeng 475004, China
| Jiejian Kou
Pharmaceutical Institute, Pharmaceutical College of Henan University, Kaifeng 475004, China
| Chunli Wang
Pharmaceutical Institute, Pharmaceutical College of Henan University, Kaifeng 475004, China
| Xiuying Yu
Agricultural College of Inner Mongolia University for Nationalities, Tongliao, 028043, China
| Xinmei Xie
Pharmaceutical Institute, Pharmaceutical College of Henan University, Kaifeng 475004, China
| Xiaobin Pang
Pharmaceutical Institute, Pharmaceutical College of Henan University, Kaifeng 475004, China
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| نشانی اینترنتی |
https://ijbms.mums.ac.ir/article_16838.html |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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