|
Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۴، صفحات ۵۰۶-۵۱۳
|
|
|
عنوان فارسی |
|
|
چکیده فارسی مقاله |
|
|
کلیدواژههای فارسی مقاله |
|
|
عنوان انگلیسی |
Identification of the effects of acid-resistant Lactobacillus caseimetallopeptidase gene under colon-specific promoter on the colorectal and breast cancer cell lines |
|
چکیده انگلیسی مقاله |
Objective(s): Anti-tumor effects of Lactobacilli as normal flora have been described. In a previous study, we identified a protein isolated from the bacterium Lactobacillus casei ATCC 39392 in acidic pH conditions named metallopeptidase. Therefore, we decided to evaluate the effect of the recombinant plasmid coding metallopeptidase protein on the inhibition, proliferation, or apoptosis of the colorectal and breast cancer cell lines. Materials and Methods: Identified metallopeptidase gene of L. casei under the specific colon cancer promoter was transferred to the Human SW480 and MDA-MB231 cells. Cell viability was evaluated in these two cancer cell lines via MTT assay, apoptotic changes, and expression level of p53 and MAP2K1 genes in comparison with healthy blood cells as a control group. Results: Viability of SW480 and MDA-MB231 cells was identified at 25% and 7%, respectively. An increase in apoptotic cell death in the SW480 cell line was observed as revealed by Tunnel staining. The expression assay of TP53 and MAP2K1 genes showed that MPL protein altered gene expression in a cell type-specific manner. Tunnel analyses showed that the pronounced cytotoxic effect of pEGFP-C2/MPL plasmid on SW480 cells was mediated through apoptosis. Conclusion: These results suggest that endogenous recombinant MPL under colon specific promoter inhibits the proliferation of SW480 colorectal cancer cells by increase in MAP2K1 and P53 activation. L. casei metallopeptidase under the same circumstances could not affect the growth rate and viability of MDA-MB231 breast cancer cells in vitro. |
|
کلیدواژههای انگلیسی مقاله |
Apoptosis Cytotoxicity Lactobacillus casei Recombinant plasmid TP53 and MAP2K1 genes, Expression |
|
نویسندگان مقاله |
| Narges Dadfarma Department of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
| Jamileh Nowroozi Department of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
| Bahram Kazemi Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Mojgan Bandehpour Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
|
|
نشانی اینترنتی |
https://ijbms.mums.ac.ir/article_17738.html |
فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
کد مقاله (doi) |
|
زبان مقاله منتشر شده |
en |
موضوعات مقاله منتشر شده |
|
نوع مقاله منتشر شده |
Original Article |
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|