چکیده انگلیسی مقاله |
Objective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. Results: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation. Conclusion: Our study implies that irisin is a potential therapeutic agent for endometritis. |
نویسندگان مقاله |
| Xi Jiang Zhejiang University Mingzhou Hospital, Ningbo, 315000, China|Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Ying Hu Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Yingjie Zhou Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Jin Chen Zhejiang University Mingzhou Hospital, Ningbo, 315000, China
| Chonglu Sun Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Ziwei Chen Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Changfeng Jing Zhejiang University Mingzhou Hospital, Ningbo, 315000, China
| Lexing Xu Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Fuhe Liu Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Wenjuan Ni Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Xuefeng Yu Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
| Lei Chen Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
|