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Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۷، صفحات ۹۱۵-۹۲۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Genotypic and phenotypic characterization of enteroaggregative Escherichia coli (EAEC) isolates from diarrheic children: An unresolved diagnostic paradigm exists |
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| چکیده انگلیسی مقاله |
Objective(s): The enteroaggregative Escherichia coli (EAEC) has been one of the most intriguing emerging bacterial pathogens in children that occur both in developing countries and the industrial world. Although various phenotypic and genotypic based protocols have been suggested for diagnosis of EAEC, they are not conclusive or practical to be used in most clinical laboratories. Materials and Methods: In this study, we analyzed and compared 36 typical EAEC strains (aggR-positive) by various genotypic and phenotypic methods.Results: Briefly, pCVD432 was detected in all of isolates along with aggR, then it was followed by other virulence genes including app, astA, aggA, and pet genes in 32 (88.8%), 21 (58.3%), 9 (25%), and 2 (5.5%) isolates, respectively. Biofilm was formed by 34 (94.4%) isolates, while only 26 (72.2%) isolates showed an aggregative adherence pattern to HEp-2 cells. Conclusion: The genetic and phenotypic features of EAEC were highly inconsistent, which may have considerable diagnostic implications. The variations in the virulence genes, phenotypic characteristics, and genetic profiles among the EAEC isolates again emphasized the genetic heterogeneity of this emerging pathotype. Biofilm formation may be an important phenotypic virulence property of this pathotype, especially in strains with the aggR-pCVD432-aap-astA profile. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
| Haiffa Helalat
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
| Seyedeh Elham Rezatofighi
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
| Mohammad Roayaei Ardakani
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
| Luis Fernando Dos Santos
Adolfo Lutz Institute, Centere of Bacteriology, National Reference Laboratory for E. coli enteric infections and HUS. São Paulo, Brazil
| Mahdi Askari Badouei
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
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| نشانی اینترنتی |
http://ijbms.mums.ac.ir/article_15495.html |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-2416906.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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