| چکیده انگلیسی مقاله |
Objective(s): Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preventing this disorder.Materials and Methods: Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, noise exposure with vehicle, noise exposure with myricetin 5 mg/Kg, and noise exposure with myricetin 10 mg/kg. All animals were sacrificed after last noise exposure. The left cochlea was dissected from each rat. It was used for mRNA expression analysis (NOX3, TGF-β1, prestin, and HSP-70). Blood samples were collected to assess superoxide dismutase (SOD) activity, 1, 1 diphenyl picrylhydrazyl (DPPH), and malondialdehyde (MDA) measurements.Results: Real time-PCR assay revealed that noise decreased NOX3 and increased TGF-β1, prestin, and HSP-70 gene expressions. Administration of myricetin at the dose of 5 mg/kg, but not at 10 mg/kg, significantly reversed these changes. Noise also increased MDA levels and decreased SOD and DPPH scavenging activities. Myricetin at the doses of 5 and 10 mg/kg also reversed these changes. Conclusion: The findings of this study showed that myricetin at the dose of 5 mg/Kg was able to reverse noise-induced abnormalities in gene expression and oxidant/anti-oxidant balance. It is a possibility that myricetin via enhancement of anti-oxidant activity induced these effects. |
| نویسندگان مقاله |
| Maryam Bahaloo
Industrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Mohammad Ebrahim Rezvani
Department of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Ehsan Farashahi Yazd
Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Fatemeh Zare Mehrjerdi
Department of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Mohammad Hossein Davari
Department of Occupational Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Ali Roohbakhsh
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
| Abolfazl Mollasadeghi
Industrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Haniyeh Nikkhah
Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Maryam Vafaei
Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
| Amir Houshang Mehrparvar
Industrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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