| چکیده انگلیسی مقاله |
Objective(s): The present study investigated the prevalence of genes encoding for exotoxins, adhesion and biofilm factors in Staphylococcus aureus isolates obtained from samples in a referral burn hospital in Tehran, Iran.Materials and Methods: S. aureus isolates obtained from patients, personnel and surfaces in the wards of a burn hospital were identified and confirmed by biochemical and molecular tests, respectively. The susceptibility of isolates was determined using the disk diffusion method. Virulence factors were detected by multiplex PCR.Results: The frequency of hla, hlb, hld, hlg, tst and pvl genes was 92.8%, 34.7%, 89.8%, 11.9%, 10.7%, and 0.5% respectively. The results revealed that the hla gene had the highest frequency among isolates (94.4% for methicillin-resistant S. aureus (MRSA) and 89.8% for methicillin-susceptible S. aureus (MSSA)). The most prevalent adhesion and biofilm-related gene was eno (85.6%). The prevalence of the remaining genes was as follows: fib (71.8%), clfB (70%), cna (59.2 %), fnbB (17.9%), icaA (72.4%), and icaD (85.6%). The incidence of fib, hlb, hlg, and tst genes was significantly higher in MRSA isolates compare to the MSSA isolates. Moreover, the resistance rates for all antibiotics were higher is MRSA isolates except for nitrofurantoin and chloramphenicol antibiotics.Conclusion: Data indicate the high prevalence rates of virulence factors among S. aureus isolates, especially MRSA strains in the burn hospital. This should to be taken into account in the development of an effective infection control policy and continuous monitoring of drug resistance in hospitals. |
| نویسندگان مقاله |
| Zahra Mir
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Narges Nodeh Farahani
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Sara Abbasian
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Faranak Alinejad
Burn Research Center, Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, Iran
| Mahboubeh Sattarzadeh
Burn Research Center, Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, Iran
| Ramin Pouriran
School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Mostafa Dahmardehei
Burn Research Center, Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, Iran
| Mehdi Mirzaii
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| Seyed Sajjad Khoramrooz
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
| Davood Darban-Sarokhalil
Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
|