Iranian Journal of Basic Medical Sciences، جلد ۲۲، شماره ۳، صفحات ۲۳۸-۲۴۵
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عنوان انگلیسی Clonal dissemination of Staphylococcus aureus isolates causing nosocomial infections, Tehran, Iran
چکیده انگلیسی مقاله Objective(s): In the current research, the prevalence of Staphylococcus aureus clones and genes encoding antimicrobial resistance and toxins were examined among 120 S. aureus strains from nosocomial infections in tehran, Iran.Materials and Methods: Antimicrobial susceptibility was examined, based on disk diffusion and PCR method to identify resistance and toxin-encoding genes. Based on the polymorphisms in SCCmec, agr, spa, and MLST, the isolates were typed. Results: Among 120 S. aureus isolates, 85 (70.8%) were  methicilin resistant S. aureus (MRSA), and 35 (29.2%) were methicilin sensetive S. aureus (MSSA). The tested isolates contained resistance genes, including ant(4΄)-Ia (90%), aac(6΄)-Ie/aph(2˝) (80%), aph(3΄)-IIIa (30%), erm(A) (26.7%), erm(B) (10.8%), erm(C) (11.7%), msr(A) (40.8%), msr(B) (14.2%), tet(M) (45.8%), and mupA (8.3%). The MRSA strains were clustered into six different clones. The most common genotypes included ST239-SCCmec III/t037 (23.3%), ST239-SCCmec III/t388 (22.5%), ST22-SCCmec IV/t790 (8.3%), ST15-SCCmec IV/t084 (7.5%), ST585-SCCmec III/t713 (5%), and ST239-SCCmec III/t924 (4.2%), respectively. ST182/t196 (8.3%) and ST123/t171 (5%) belonged exclusively to MSSA strains. Overall, 10 (66.7%) and 5 (33.3%) out of 15 isolates with pvl genes were attributed to clones ST22-SCCmec IV/t790 and ST15-SCCmec IV/t084, respectively. ST22-SCCmec IV/t790, ST239-SCCmec III/t037, and ST15-SCCmec IV/t084, were related to high-level mupirocin-resistant phenotypes.  Conclusion: The genetic diversity of S. aureus was confirmed in our hospitals, and ST239-SCCmec III/t037 showed a relatively high prevalence in our study. It seems that assessment of resistance and virulence genes in different S. aureus molecular types is necessary for proper antibiotic consumption.
کلیدواژه‌های انگلیسی مقاله Staphylococcus aureus, SCCmec, agr, MLST, MRSA, spa
نویسندگان مقاله | Mehdi Goudarzi
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Gita Eslami
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Razieh Rezaee
Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran


| Mohsen Heidary
Department of Medical Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran


| saeed khoshnood
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Raheleh Sajadi Nia
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_12031.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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