Iranian Journal of Basic Medical Sciences، جلد ۲۲، شماره ۲، صفحات ۱۲۸-۱۳۳
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عنوان انگلیسی Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells
چکیده انگلیسی مقاله Objective(s): It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Materials and Methods: Primary cultured RPE cells were treated with ELF-PEMF (50 Hz) for three days. Using ELISA assay, we evaluated the effects of treatment on RPE cell proliferation and apoptosis. Also, RT-PCR was used to determine the gene expression of proangiogenic factors, such as matrix metalloproteinase-2 (MMP-2), MMP-9, vascular endothelial growth factors receptor 2 (VEGFR-2), hypoxia-inducible factor 1 (HIF-1α), VEGFA, cathepsin D, connective tissue growth factor (CTGF), E2F3, tissue inhibitors of metalloproteinases 1 (TIMP-1), and TIMP-2.Results: No noticeable changes were observed in cell proliferation and cell death of ELF-PEMF-exposed RPE cells, while transcript levels of proangiogenic genes (HIF-1α, VEGFA, VEGFR-2, CTGF, cathepsin D, TIMP-1, E2F3, MMP-2, and MMP-9) increased significantly.Conclusion: RPE cells are important for homeostasis of the retina. ELF-PEMF increased the gene expression of proangiogenic factors in RPE cells, which highlights concerns about the impact of this treatment on human health.
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نویسندگان مقاله | Morteza Oladnabi
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran|Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran


| Abouzar Bagheri
Department of Clinical Biochemistry and Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Mozhgan Rezaei Kanavi
Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Abbas Azadmehr
Immunology Department, Babol University of Medical Sciences, Babol, Iran


| Anvarsadat Kianmehr
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran|Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_12011.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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