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Iranian Journal of Immunology، جلد ۲، شماره ۲، صفحات ۱۰۳-۱۱۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Level of Nitric Oxide in Bronchoalveolar Lavage Fluid of Asthmatic Mice Model |
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| چکیده انگلیسی مقاله |
Background: Asthma is a chronic inflammatory disease with multifactorial and complicated mechanisms. Elevated level of exhaled Nitric Oxide (NO) in asthma and other inflammatory lung diseases has led to many studies examining NO as a potential marker of airway inflammation. Objective: This study was designed to determine the level of NO in Bronchoalveolar Lavage (BAL) fluid during early and late stages of asthmatic attack in mouse model. Methods: In this study male BALB/c mice were used. The level of NO was determined in BAL fluid of asthmatic mice five minutes, six and sixteen hours after challenge with methacholine, as irritant and smoke and 5% ovalbumin as allergens, using colorimetric assay. Results: The level of NO increased upon exposure to all three irritants used in this study (52.3 μM for smoke and 49.5 μ Mfor methacholine) as compared to 22.8 μM for the baseline. Our results showed that NO levels were increased during early phase of asthmatic condition and reached to its maximum level after six hours and decreased at the late stage of asthma (16hrs) possibly by activating a feedback regulatory loop. In addition, high level of NO led to the hypertrophy of smooth muscle that can account for the pathological changes associated with asthma. Conclusion: Thus, NO is an inflammatory marker in asthma and its measurement, as a non-invasive method during asthmatic attack is suggested. A careful development of specific inhibitors for iNOS enzyme during asthmatic attack is also necessary. |
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| کلیدواژه‌های انگلیسی مقاله |
asthma,BAL fluid,Inflammation,Methacholine,NO,Ovalbumin |
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| نویسندگان مقاله |
علیرضا سالک مقدم | salek moghaddam
department of immunology
محمد شعبانی |
department of biochemistry, iran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
فرحدخت فاطمی نسب |
department of immunology
محمدرضا خاکزاد | mohammad reza
department of immunology
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| نشانی اینترنتی |
http://iji.sums.ac.ir/article_16848_94c229943dbf8bf0390d2987226c4fe7.pdf |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
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