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Iranian Journal of Immunology، جلد ۵، شماره ۴، صفحات ۲۰۷-۲۱۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects |
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| چکیده انگلیسی مقاله |
Background: HLA-G gene contains 15 alleles including a null allele, HLA-G*0105N. Previous studies have shown that HLA-G*0105N does not encode the complete HLA-G1 or HLA-G5 isoforms but encodes a functional HLA-G protein with the ability to in-hibit NK cell cytolysis. Thus, although the biological functions of HLA-G1 and HLA-G5 proteins are abrogated, other isoforms such as HLA-G2 can replace their roles. Stud-ies on the null allele of HLA-G gene could be useful in understanding the genetic vari-ants of HLA-G alleles in ethnic groups. Objective: The goal of this research was to de-termine the frequency of HLA-G*0105N null allele in Iranian healthy subjects. Meth-ods: The frequency of HLA-G*0105N null allele was evaluated in Iranian healthy sub-jects by PCR-RFLP method. Genomic DNA was isolated from the whole blood of 100 randomly selected, healthy, unrelated Iranian individuals using salting-out technique followed by PCR amplification of the exon 3 of HLA-G gene. PCR products were di-gested with PpUM-1 and the resulted fragments were analyzed using gel electrophore-sis. Results: In this study the restriction enzyme digestion confirmed homozygous HLA-G*0105N null allele for 9 % of the population. Furthermore obtained results indi-cated that the total frequency of HLA-G*0105N null allele was 20 % in the studied population of Iran. Conclusion: The final data analysis showed that the total frequency of this allele in Iranian people was higher than other ethnic groups that have been stud-ied so far. |
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| کلیدواژه‌های انگلیسی مقاله |
Frequency,HLA-G*0105N antigen,Iran,RFLPs |
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| نویسندگان مقاله |
رقیه رحیمی |
tarbiat modares university, school of medical sciences
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
احمد زواران حسینی | zavaran hosseini
tarbiat modares university, school of medical sciences
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
فاطمه یاری |
research center, iranian blood transfusion organization, tehran, iran
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
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| نشانی اینترنتی |
http://iji.sums.ac.ir/article_17169_3ec26db0c327dcd23463357af63dd097.pdf |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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