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Iranian Journal of Immunology، جلد ۸، شماره ۳، صفحات ۱۵۰-۱۵۸
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عنوان انگلیسی Validation of a β-ME ELISA for Detection of Anti Leishmania donovani Antibodies in Eastern Sudan
چکیده انگلیسی مقاله Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-DAT) and rK39 strip test (RKT) commercial kits. Methods: Two-hundred and ninety-two sera from patients with high VL suspicion of whom 105 had confirmed L. donovani infection were tested. Results: Relatively higher sensitivities of 93.3% (95% CI: 88.4- 98.2) and 92.4% (95% CI: 87.3-97.5) were determined for β-ME ELISA and FD-DAT as compared to 83.8% (95% CI: 76.7-90.8) for RKT. Of 73 VL sera that scored maximum absorbance values (>0.81) in β-ME ELISA, 66 (90.4%) tested at the highest agglutination titres (>1:51200) in FD-DAT as did 56 (76.7%) also at comparable reaction intensities (3 + colour intensity) in RKT. Compared with FD-DAT (94.7%, 95% CI: 91.5-97.9) or RKT (93.0%, 95% CI: 89.3-96.6), lower specificity was estimated for β-ME ELISA (90.4%, 95% CI: 86.1-94.6). Based both on positive and negative microscopy for L. donovani in organ aspirates of all VL suspects enrolled (292), significantly higher correlation (p
کلیدواژه‌های انگلیسی مقاله β-ME,DAT,ELISA,rK39 Antigen,visceral leishmaniasis
نویسندگان مقاله elfadil عباس |
biomedical research laboratory, ahfad university for women, omdurman, sudan

abdelhafeiz محمود |
biomedical research laboratory, ahfad university for women, omdurman, sudan

durria منصور |
biomedical research laboratory, ahfad university for women, omdurman, sudan

مهدی محبعلی |
department of medical parasitology and mycology, school of public health, university of medical sciences, tehran, iran

عبدالله el harith | abdollah el
professor emeritus of microbiology, ahfad university for women, omdurman, sudan

نشانی اینترنتی http://iji.sums.ac.ir/article_17020_7438dfde8c9ecd1b19d8ae54487b77cf.pdf
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