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Iranian Journal of Microbiology، جلد ۱، شماره ۲، صفحات ۵۴-۶۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Characterization of novel biosurfactant producing strains of Bacillus spp. isolated from petroleum contaminated soil |
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| چکیده انگلیسی مقاله |
Background and objectives: Biosurfactants are surface active agents with broad range commercial applications in various industries and have considerable advantages over their chemical counterparts. Materials and Methods: In this study, bacteria were isolated from contaminated and uncontaminated soil and selected during preliminary screening using hemolytic activity, oil spreading and oil collapsed techniques. Isolates with at least more than one positive response to these three methods were subjected to complementary screening by measuring surface tension reduction as well as emulsification capacity. The criteria for selection of potent isolates were surface tension reduction below 40 mN/m and emulsification capacity of more than fifty percent. Results: Using these stepwise screening procedures, two biosurfactant/bioemulsifier producing isolates have been successfully selected that were able to reduce surface tension effectively and one of which formed a stable emulsion. Conclusion: Phylogenic relationships of the two potential candidates were determined comparing the 16Sr DNA gene sequences, revealing them as two isolates of Bacillus subtilis and Bacillus cereus that can be used in pilot scale for industrial production of new biosurfactant/bioemulsifier. |
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| کلیدواژه‌های انگلیسی مقاله |
Bacillus,biosurfactant,emulsification,petroleum,soil |
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| نویسندگان مقاله |
s نصر | s nasr
department of microbiology, faculty of sciences, alzahra university,tehran, iran.
سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)
m r صعودی | m r soudi
department of microbiology, faculty of sciences, alzahra university,tehran, iran.
سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)
m r مهرنیا | m r mehrnia
biotechnology and environment group, department of chemical engineering, college of engineering,tehran, iran.
m h صراف زاده | m h sarrafzadeh
biotechnology and environment group, department of chemical engineering, college of engineering,tehran, iran.
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/view/19 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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