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Iranian Journal of Microbiology، جلد ۱، شماره ۴، صفحات ۳۲-۳۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی A molecular survey of Avian Influenza among captive birds in the city of Tehran between November 2008 and February 2009
چکیده انگلیسی مقاله Background and objectives: To determine the potential circulation of avian influenza viruses among different captive bird species, molecular surveillance was conducted at Tehran Zoo, Saiee Park and Pardisan Park of Tehran, Iran. These places are at risk for spread and transmission of influenza virus because of bird species diversity and close contact of birds with humans. Materials & Methods: During the influenza season in Tehran, in the cold weather (November 2008-February 2009), 76 cloacae samples were collected from 5 orders of Anseriformes, Galliformes, Columbiformes, Pelicaniformes and Phoenicopteriformes, including 13 bird species plus 5 hybrid species of ducks. Presence of avian influenza genome was monitored with RT-PCR as a sensitive and specific assay. The assay targeted a 132 bp fragment of the conserved M gene of influenza type A. Results: Influenza type A virus was not detected in samples collected from November 2008 to February 2009. The sensitivity of RT-PCR based on M primers was 0.1ng total RNA. Interestingly, during the study period, there was no report of death or clinical signs of disease among the c aptive birds, whereas the birds did not have vaccinated history against influenza A virus. Conclusion: Although the results could be attributed at least partially to the presence of an undetectable amount of genomic RNA, based upon the sensitivity of the test our findings suggest that no RNA genome of influenza A viruses was present in the samples under study.
کلیدواژه‌های انگلیسی مقاله Avian influenza viruses,RT-PCR,Captive birds,Tehran
نویسندگان مقاله سید مسعود حسینی | seyed masoud hosseini
department of microbiology, faculty of biological science, shahid beheshti university, evin, tehran,iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

b حیدرچی | b heydarchi
department of microbiology, faculty of biological sciences, shahid-beheshti university, tehran,iran and inf luenza unit, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

n شیخی | n sheykhi
department of clinical sciences, facult y of specialized veterinary science, science and research campus, islamic azad university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

m طباطباییان | m tabatabaian
inf luenza unit, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

r بشر | r bashar
influenza unit, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

sf فهیمه موسوی | sf fahime mousavi
department of microbiology, faculty of biological sciences, shahid-beheshti university, tehran,iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

mt خیری | mt kheiri
influenza unit, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/37
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