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Iranian Journal of Microbiology، جلد ۲، شماره ۱، صفحات ۳۳-۴۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Optimization and comparative characterization of neuraminidase activities from Pseudomonas aeruginosa with Klebsiella pneumoniae,Hep-2 cell, sheep kidney and rat liver lysosome |
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| چکیده انگلیسی مقاله |
Background and Objectives: The properties of neuraminidase produced by P. aeruginosa strain PAO1 during growth in a defined medium (BHI) was examined and compared with some neuraminidase features of K. pneumoniae in this investigation. Materials and Methods: The enzyme was isolated from concentrated culture supernatants of P. aeruginosa which was used in a sensitive fluorometric assay by using 2'-(4-methylumbelliferyl) α-D-N acetylneuraminic acid as substrate. Results: Neuraminidase production in P. aeruginosa PAO1 paralleled bacterial growth in defined medium (BHI) and was maximal in the late logarithmic phase of growth but decreased during the stationary phase, probably owing to protease production or thermal instability. Highest production of P. aeruginosa PAO1 neuraminidase was in BHI culture media. The neuraminidase of P. aeruginosa PAO1 possessed an optimum temperature of activity at 56 °C and the activity was maximal at pH 5. Heating the enzyme to 56 °C for 45 min., in the presence of bovine serum albumin destroyed 33.1% of it's activity and addition of Ca+2, EDTA and NANA also decreased activity markedly. Conclusion: The results revealed that the highest specific activity is for p. aeruginosa PAO1. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
c قضایی | c ghazaei
department of microbiology, faculty of veterinary medicine, university of urmia, urmia, iran.
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)
احمدی | m ahmadi
department of microbiology, faculty of veterinary medicine, university of urmia, urmia, iran.
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)
n حسینی جزنی | n hosseini jazani
2department of microbiology, immunology and genetics, faculty of medicine, urmia university of medical sciences, urmia, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/view/45 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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