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Basic and Clinical Cancer Research، جلد ۶، شماره ۱، صفحات ۱۶-۲۱
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عنوان انگلیسی The Study of LPL Gene Expression during Differentiation of Human BM-MSC into Adipocytes
چکیده انگلیسی مقاله Background : Many types of cancer cells require a supply of fatty acids (FA) for growth and survival, LPL, in the presence of triglyceride-rich lipoproteins, accelerates the growth of these cells. Recent evidence shows that LPL plays important roles in inflammation and obesity, implies that it is an appropriate general target for chemo preventive and chemotherapeutic agents. Thus, we reported LPL expression before and after BM-MSCs differentiation into adipocytes. Methods : In this study, we isolated mesenchymal stem cells from human bone marrow by Ficoll - gradient and then their surface markers by flow cytometry confirmed and osteoblastic and adipocytes differentiation by Dexamethasone protocol were done and confirmed by staining .Then qualitative expression of LPL gene by RT-PCR before and after of differentiation into adipocytes .Statistical analysis was performed by paired t test and by using Pfaffl and graph pad software . Results : After 14 days, analysis of morphology with invert microscopy indicated that BM-MSCs have ability of differentiation capacities into adipocytes. Investigations of expression of LPL showed BM-MSC that served as negative control have not expression of LPL while in BM-MSCs derived- adipocyte have found significant expression of LPL. Conclusions: BM-MSCs can differentiate into either adipocytes or osteoblastic cells that in decision between the two lineages, LPL has the important role through activation inhibiting osteoblastic in favor of adipogenic differentiation. LPL could be an effective agent on tumor suppressing with catalyzes the hydrolysis of plasma TG.  LPL gene deficiency, such as due to chromosome 8p22 loss, LPL gene polymorphism, and epigenetic changes increases cancer risk, especially in the prostate.
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نویسندگان مقاله سید رسول رضوی باباحیدری | seied rasoul razavi babaheidari
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

کامران موسوی حسینی | kamran mousavi hosseini
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

امیر آتشی | amir atashi
department of hematology, school of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

آرزو اودی | arezou oodi
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

شادی اسماعیلی | shadi esmaeili
department of hematology, school of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://bccr.tums.ac.ir/index.php/bccrj/article/view/132
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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